TY - JOUR ID - 43483 TI - Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Atabi, Fereshteh AU - Mohammadi, Reza AD - Department of Biochemistry and Biophysics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran AD - Department of Biochemistry, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Y1 - 2020 PY - 2020 VL - 15 IS - 4 SP - 261 EP - 267 KW - cardiovascular disease KW - LDL KW - Friedewald formula KW - HDL KW - TG DO - 10.30699/ijp.2020.110379.2174 N2 - Background & Objective: Concentration of low-density lipoprotein (LDL) is a known risk factor for cardiovascular disease which is routinely measured or calculated as LDL-C in clinical laboratories. In order to decrease the cost, instead of its measuring, it is recommended to calculate it using multiple formulas that have been introduced up to now. The aim of this study was to assess the results of various formulas and comparison of these results with those of measuring method and to clarify the best formula for the Iranian population. Methods: Concentrations of total cholesterol (TC), triglyceride (TG), cholesterol of high-density lipoprotein (HDL-C) and LDL-C in serums of 471 overnight fasting individuals were measured and also LDL-Cs of these samples were calculated by eleven different formulas according to their TC, TG, and HDL-C concentrations. Subsequently, results of measured and calculated LDL-C were analyzed statistically by paired t-test, correlation coefficient, and Passing-Bablok regression. In addition, for clinical evaluation, the differences between calculated and measured mean results were calculated and compared with an allowable total error. Result: Paired t-test unraveled a significant difference between the results of measured and calculated LDL-C by various formulas. But for some formulas, these differences were not clinically significant. The best clinical and statistical agreement (correlation coefficient) was obtained by the Friedewald equation. Conclusion: By using validated methods which have correct calibration and control system for measuring TC, TG, and HDL-C, we can use the Friedewald formula for calculating LDL-C in serum samples with TG up to 400 mg/dL. UR - https://ijp.iranpath.org/article_43483.html L1 - https://ijp.iranpath.org/article_43483_ad6be4eae7271d961529cd7304f66110.pdf ER -