TY - JOUR ID - 32427 TI - Peripheral Blood-Derived Mesenchymal Stem Cells: Growth Factor-Free Isolation, Molecular Characterization and Differentiation JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Pouryazdanpanah, Noushin AU - Dabiri, Shahriar AU - Derakhshani, Ali AU - Vahidi, Reza AU - Farsinejad, Alireza AD - Dept. of Hematology and Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran AD - Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran AD - Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran AD - Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran AD - Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran Y1 - 2018 PY - 2018 VL - 13 IS - 4 SP - 461 EP - 466 KW - Peripheral blood KW - Mesenchymal stem cells KW - Differentiation KW - Regenerative medicine DO - N2 - Background and Objectives: The mesenchymal stem cells derived from peripheral blood (PB) have been recognized as a promising source for allogeneic cell therapy. The aim of this study was to investigate the isolation, growth and differentiation ability of peripheral blood-isolated mesenchymal stem cells. Methods: The mononuclear cells were purified from fresh peripheral blood using density gradient centrifugation then cultured in a suitable medium, expanded and characterized. In the following, these cells were cultured in specific adipogenic and osteogenic differentiation media.Results and Conclusion: In spite of the absence of any stimulating factor, the cells adhered to the flasks and developed a rather homogeneous, spindle-shaped morphology after consecutive passages. The cells were confirmed to have mesenchymal phenotype by expression of specific markers (CD90, CD105, and CD73) and absence of CD45 marker, which is specific for hematopoietic stem cells. They could differentiate into lineage-specific committed cells (osteoblasts and adipocytes). According to the findings, the conventional, labour-intensive and time-consuming approaches are not necessary to obtain an optimal number of cells from peripheral blood. This relatively accessible and minimally invasive source of stem cells may open a new era for practical exploitation in regenerative medicine. UR - https://ijp.iranpath.org/article_32427.html L1 - https://ijp.iranpath.org/article_32427_6b3248f745cda5f1e270fa888cc7c9e3.pdf ER -