TY - JOUR ID - 32422 TI - MiR-21 Expression in Wilms’ Tumor JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Bazzaz, Navid AU - Nouraee, Nazila Nouraee AU - Zare-Mirzaie, Ali AU - Mowla, Seyed Javad Mowla AU - Shahali, Maryam AU - Vasei, Mohammad AD - Tehran university of medical sciences, Tehran, Iran AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran, Department of Experimental Therapeutics, MD Anderson Cancer Center, University of Texas, Houston, Texas, AD - Fellow of molecular pathology and cytogenetic, Tehran University Medical Sciences, Tehran, Iran. Associate professor of pathology, Iran University Medical Sciences, Tehran, Iran AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University,Tehran, Iran AD - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran AD - Cell Based Therapies Research Center , Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran Y1 - 2018 PY - 2018 VL - 13 IS - 4 SP - 422 EP - 428 KW - Wilms’ tumor KW - miR-21 KW - CISH KW - Real-time PCR DO - N2 - Background and Objective: Wilms’ tumor (WT) is the most common genitourinary tract tumor in children. MicroRNAs (miRNAs) are small non-coding RNAs; their role in the pathogenesis of many types of human cancers has been identified. We aimed to evaluate the expression of miR-21, a well-known oncomir, in WT tissue samples which is a very common urinary tract malignancy in children. Methods: We performed chromogenic in situ hybridization (CISH) to detect the sub-cellular localization of miR-21 in 25 formalin-fixed, paraffin-embedded (FFPE) samples of WT. We also evaluated miR-21 expression in 24 of these blocks and 6 normal kidneys as controls using quantitative real-time PCR technique. Results: While our real-time PCR analysis showed miR-21 significant overexpression in 4 tumors compared to the normal kidney samples, we could not detect significant ISH signal in any of these samples. Conclusion: Low expression of miR-21 in WT might pinpoint the weak involvement of this miRNA in the pathogenesis of this cancer. UR - https://ijp.iranpath.org/article_32422.html L1 - https://ijp.iranpath.org/article_32422_a2f438059f5e501f8c4521fb9997b722.pdf ER -