TY - JOUR ID - 24875 TI - Conserved OprF as a Selective Immunogen Against Pseudomonas aeruginosa JO - Iranian Journal of Pathology JA - IJP LA - en SN - 1735-5303 AU - Kazemi Moghaddam, Ehsan AU - Owlia, Parviz AU - Jahangiri, Abolfazl AU - Rasooli, Iraj AU - Rahbar, Mohammad Reza AU - Aghajani, Marjan AD - Dept. of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran. AD - Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran. AD - Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. AD - Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran. AD - Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Dept. of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Y1 - 2017 PY - 2017 VL - 12 IS - 2 SP - 165 EP - 170 KW - OpF Protein KW - Pseudomonas aeruginosa KW - Vaccine DO - 10.30699/ijp.2017.24875 N2 - Background & Objectives: Due to the importance of Pseudomonas aeruginosa in severe inpatient infections and high mortality, the need for an efficient vaccine against these bacteria is increasing. In this regard, the general outer membrane porin of the most problematic microorganism P. aeruginosa, outer membrane protein F (OprF), is a good vaccine candidate. Methods:The databank of NCBI was used to retrieve protein sequences recorded for OprF in P. aeruginosa.The current study aimed at investigating the conservation of the OprF in 150 reference sequences, clinical, and environmental strains of P. aeruginosa from different countries via bioinformatic tools.T-COFFEE and PRALINE software were used for alignment. Results: Of these, 134 strains were isolated from clinical specimens and other strains from environmental samples. Evaluation of alignment by the mentioned software clearly showed that this protein was conserved. Antigenicity and grand average of hydropathicity were favorable. Conclusion: Conservation of OprF in all pathogenic and environmental strains of P. aeruginosa indicated that it can be considered as a good immunogen; however, the protectivity of OprF should be validated experimentally. UR - https://ijp.iranpath.org/article_24875.html L1 - https://ijp.iranpath.org/article_24875_a80a057b07cff66378af702de6d09961.pdf ER -