Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Herpes Simplex Virus and Langerhans Cell Histiocytosis
323
328
EN
Maliheh
Khoddami
0000000259434458
Pediatric Pathology Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
malihehkhoddami@yahoo.com
Seyed Alireza
Nadji
Virology Research Center (VRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
s.a.nadji@sbmu.ac.ir; sarnadji@yahoo.com
Paria
Dehghanian
Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
paria_email@yahoo.com
10.30699/ijp.2017.27988
<strong><em>Background and objective: </em></strong>Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferative disorder of unknown etiology and mainly affects young children. The histological feature is granuloma-like proliferation of langerhans-type dendritic cells. Although the possible role of viruses such as <em>Epstein-Barr virus </em>(<em>EBV</em>, <em>Human Herpes virus -4</em>), <em>Human Herpes virus-6 </em>(<em>HHV-6</em>), <em>Herpes Simplex virus</em> (<em>HSV</em>) types 1 and 2 and <em>Cytomegalovirus</em> (CMV, <em>Human Herpes virus-5</em>) is suggested in the pathogenesis of LCH by some investigators, its exact pathophysiology has not been cleared yet. In this study, we investigated the presence of <em>HSV</em> types 1 and 2 in Iranian children with LCH<br /> <strong><em>Methods: </em></strong>In this retrospective study, we investigated the prevalence of presence of <em>HSV</em> types 1 and 2 (in 30 patients with LCH), using paraffin-embedded tissue samples and 30 age and tissue-matched controls (operated for reasons other than infectious diseases) from the Department of Pediatric Pathology, Tehran, Iran, by nested Polymerase Chain reaction method. No ethical issues arose in the study, because only the pathology reports were reviewed and patients were anonymous.<br /> <strong><em>Results: </em></strong>We failed to find <em>HSV</em> types 1 and 2 DNA in any of the 30 patients with LCH or the control group.<br /> <strong><em>Conclusion:</em> </strong>According to our findings, <em>HSV</em> types 1 and 2 do not appear to have any etiologic role in the pathogenesis of LCH in Iranian children. These results are in accordance with previous investigations with negative findings.
Histiocytosis, Langerhans-Cell,Herpes Simplex Virus, Polymerase Chain reaction,Cell proliferation
https://ijp.iranpath.org/article_27988.html
https://ijp.iranpath.org/article_27988_921b1ae56168c39dd7deb653eb94fc3e.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran
329
338
EN
Mehrdad
Gholami
Dept. of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
mehrdad_gholami90@yahoo.com
Mohammadreza
Haghshenas
Dept. of Microbiology, Molecular and Cell-Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
haghshenas2001@yahoo.com
Mona
Moshiri
0000-0001-8594-0496
Dept. of Pathobiology, Division of Microbiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
m_moshiri91@yahoo.com
Shbnam
Razavi
Dept. of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
razavi.sh@iums.ac.ir
Abazar
Pournajaf
Dept. of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
abazar_pournajaf@yahoo.com
Gholamreza
Irajian
Dept. of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
dr.irajian@gmail.com
Mohsen
Heidary
Dept. of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
mohsenheidary40@gmail.com
10.30699/ijp.2017.27989
<strong><em>Background & objective</em></strong><strong>:</strong> Multidrug-resistant <em>Acinetobacter baumannii</em> (MDR-AB) is an important nosocomial pathogen which is associated with significant morbidity and mortality, particularly in high-risk populations. Aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (16S rRNA) methylation are two important mechanisms of resistance to aminoglycosides. The aim of this study was to determine the prevalence of 16S rRNA methylase (<em>armA</em>, <em>rmtA</em>, <em>rmtB</em>, <em>rmtC</em>, and <em>rmtD</em>), and the AME genes [<em>aac(6′)-Ib</em>, <em>aac(3)-I</em>, <em>ant(3′′)-I</em>, <em>aph(3′)-I</em> and <em>aac(6')-Id</em>], among clinical isolates of <em>A. baumannii</em> in Tehran, Iran.<br /> <strong><em>Methods</em></strong><strong>:</strong> Between November 2015 to July 2016, a total of 110 clinical strains of <em>A. baumannii</em> were isolated from patients in two teaching hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute guidelines. The presence of genes encoding the AMEs and16S rRNA methylases responsible for resistance was investigated by multiplex polymerase chain reaction.<br /> <strong><em>Results</em></strong><strong>:</strong> The results showed that colistin was an effective antibiotic and could be used as a last-resort treatment of infections caused by MDR-AB<strong>. </strong>The resistance rate to aminoglycosides were 100%, 96.36% and 90.9% for tobramycin, gentamicin and amikacin, respectively. In this study, AME genes of <em>aac(6′)-Ib</em>, <em>aac(3)-I</em> and <em>ant(3′′)-I</em> were most prevalent among the isolated strains.<br /> <strong><em>Conclusion</em></strong> Markedly high resistance to tobramycin, gentamicin and amikacin was noted in current study. Our results suggested that modifying enzyme genes in conjunction with methylation of 16S rRNA might contribute to aminoglycoside resistance induced in vivo in <em>A. baumannii</em>.Further studies are required to determine the prevalence of the aminoglycoside resistance genes in other hospitals of Iran.
Acinetobacter baumannii,Multidrug resistance,Aminoglycoside-modifying enzymes, 16S rRNA methylase
https://ijp.iranpath.org/article_27989.html
https://ijp.iranpath.org/article_27989_c38e0552199637046707323a99e15c57.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Classification and Biomarker Genes Selection for Cancer Gene Expression Data Using Random Forest
339
347
EN
Malihe
Ram
Dept. of Biostatistics, Public Health School, Mashhad University of Medical Sciences, Mashhad, Iran
rammalihe@yahoo.com
Ali
Najafi
0000-0002-2540-4955
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
najafi74@yahoo.com
Mohammad Taghi
Shakeri
Dept. of Biostatistics, Public Health School, Mashhad University of Medical Sciences, Mashhad, Iran
shakerimt@mums.ac.ir
10.30699/ijp.2017.27990
<strong><em>Background & objective</em></strong><strong>: </strong>Microarray and next generation sequencing (NGS) data are the important sources to find helpful molecular patterns. Also, the great number of gene expression data increases the challenge of how to identify the biomarkers associated with cancer. The random forest (RF) is used to effectively analyze the problems of large-p and small-n. Therefore, RF can be used to select and rank the genes for the diagnosis and effective treatment of cancer.<br /> <strong><em>Methods: </em></strong>The microarray gene expression data of colon, leukemia, and prostate cancers were collected from public databases. Primary preprocessing was done on them using limma package, and then, the RF classification method was implemented on datasets separately in R software. Finally, the selected genes in each of the cancers were evaluated and compared with those of previous experimental studies and their functionalities were assessed in molecular cancer processes.<br /> <strong><em>Result: </em></strong>The RF method extracted very small sets of genes while it retained its predictive performance. About colon cancer data set <em>DIEXF</em>, <em>GUCA2A</em>, <em>CA7</em>, and <em>IGHA1</em> key genes with the accuracy of 87.39 and precision of 85.45 were selected. The <em>SNCA</em>, <em>USP20</em>, and <em>SNRPA1</em> genes were selected for prostate cancer with the accuracy of 73.33 and precision of 66.67. Also, key genes of leukemia data set were <em>BAG4</em>, <em>ANKHD1</em>-<em>EIF4EBP3</em>, <em>PLXNC1</em>, and <em>PCDH9</em> genes, and the accuracy and precision were 100 and 95.24, respectively.<br /> <strong><em>Conclusion:</em></strong> The current study results showed most of the selected genes involved in the processes and cancerous pathways were previously reported and had an important role in shifting from normal cell to abnormal.
Microarray,Random Forest,Cancer,Gene Selection,Classification
https://ijp.iranpath.org/article_27990.html
https://ijp.iranpath.org/article_27990_2b14f68527d9aec085a93dbc82633079.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Association of Candida sp. with the Degrees of Dysplasia and Oral Cancer: A Study by Calcofluor White under Fluorescent Microscopy
348
355
EN
Sandhya
Tamgadge
Department of Oral and Maxillofacial Pathology and Microbiology, D .Y. Patil Dental College, school of dentistry l Sector 7, NerulNavi Mumbai , Maharashtra , India
sandhya.tamgadge@gmail.com
Avinash
Tamgadge
Department of Oral and Maxillofacial Pathology and Microbiology, D .Y. Patil Dental College, school of dentistry l Sector 7, NerulNavi Mumbai , Maharashtra , India
avinash.pt@gmail.com
Aswathy
Pillai
Department of Oral and Maxillofacial Pathology and Microbiology, D .Y. Patil Dental College, school of dentistry l Sector 7, NerulNavi Mumbai , Maharashtra , India
pillai.aswathy80@gmail.com
Mayura
Chande
Department of Oral and Maxillofacial Pathology and Microbiology, D .Y. Patil Dental College, school of dentistry l Sector 7, NerulNavi Mumbai , Maharashtra , India
mayurachande1234@gmail.com
Siddharth
Acharya
Department of Oral and Maxillofacial Pathology and Microbiology, D .Y. Patil Dental College, school of dentistry l Sector 7, NerulNavi Mumbai , Maharashtra , India
siddharth.acharya@gmail.com
Narayan
Kamat
Department of Oral and Maxillofacial Pathology and Microbiology, D .Y. Patil Dental College, school of dentistry l Sector 7, NerulNavi Mumbai , Maharashtra , India
nktallur@gmail.com
10.30699/ijp.2017.27991
<strong><em>Background and objective:</em></strong><em>Candida albicans</em> (<em>C. albicans</em>) play a significant role in oral mucosal carcinogenesis. It can be identified using various techniques in cytological smears. But, very few studies have been conducted on histopathological sections using calcofluor white M2R under fluorescent microscopy. Additionally, detection and quantification of <em>Candida</em> colonies and its correlation with various grades of oral leukoplakia and oral carcinomas have not been explored much.<br /> <strong><em>Methods:</em></strong>The current retrospective study included 80 samples from archives consisting of 60 samples in the study group (10 cases each of mild, moderate, and severe epithelial dysplasia (totally 30) and 30 cases of oral carcinoma). Sections were stained with calcofluor white (CFW) and 10% KOH for the observation under fluorescent microscopy and correlated with different grades of oral leukoplakia and oral carcinomas. Chi-square test was used in SSPS software to study the presence and absence of <em>Candida</em> sp. in different groups.<br /> <strong><em>Results:</em></strong>The study groups of oral carcinoma and dysplasia showed a significant association with <em>Candida</em> sp. (P=0). When carcinoma was compared with each grade of dysplasia, except mild dysplasia (P=4.4E-05), both moderate (P=0.402195) and severe dysplasia (P=0.558746) showed an insignificant P-value. When the groups of mild (13.3%), moderate (30%), and severe (33.3%) dysplasia were considered independently, the incidence of <em>Candida</em> sp. increased as the grade of dysplasia increased. The number of colonies have been counted and the maximum number of colonies have been observed in carcinoma and the least have been observed in mild dysplasia.<br /> <strong><em>Conclusion:</em> </strong>A significant association of <em>Candida</em> colonies with epithelial dysplasia and oral cancer was established. Further, CFW was found a promising candidate to identify <em>Candida</em> colonies in tissue sections using fluorescent microscopy.
Candida albicans,Calcoflour White,Fluorescent Microscopy,Histopathology,Leukoplakia,Dysplasia,Oral carcinoma
https://ijp.iranpath.org/article_27991.html
https://ijp.iranpath.org/article_27991_1b2ef2b31d4a523ba9b79039ae584333.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Bacterial Etiology and Antibiotic Resistance Patterns in Neonatal Sepsis in Tehran during 2006-2014
356
361
EN
Fatemeh
Haj Ebrahim Tehrani
Dept. of Neonatology, Shahed Medical Faculty, Shahed University, Tehran, Iran
khayat@shahed.ac.ir
Mohammad
Moradi
0000-0003-3653-5739
Students Research Committee of Shahed Medical Faculty, Shahed University, Tehran, Iran
mohammad.mrdi@gmail.com
Narjes
Ghorbani
Shahed Medical Faculty, Shahed University, Tehran, Iran
narjesghorbani@yahoo.com
10.30699/ijp.2017.27992
<strong><em>Background & Objective</em></strong><strong>: </strong>Neonatal sepsis is one of the leading causes of mortality in neonatology wards. The aim of this study was to assess sepsis pathogens and antibacterial resistance patterns in a teaching hospital during seven years in Tehran, Iran.<br /> <strong><em>Methods:</em></strong> In this retrospective study, all neonates suspected to sepsis and fulfilling the sepsis criteria admitted to NICU ward of Mustafa Khomeini Hospital, Tehran, Iran during 2007 to 2014 were included. Demographic information, blood test results, blood culture results of neonates and antibiogram findings were extracted from their documents. Data was analyzed using SPSS 15.<br /> <strong><em>Results:</em></strong>Ninety neonates with positive culture test were included. Fifty-three were male (58.9%). Thirty neonates were delivered vaginally (33.3%) and 60 caesarean section (66.7%). Most bacterial growths in culture were <em>Staphylococcus aureus</em> and <em>E. coli</em>. The rates of resistance for antibiotics like ceftriaxone, cefotaxim and gentamycin were 5%, 30% and 15%, correspondingly. There were 15 cases (16.7%) with resistance to imipenem.<br /> <strong><em>Conclusion:</em> </strong>Antibacterial resistance patterns vary in different parts of the world and even within a country, therefore assessing resistance patterns in a region is of great importance for proper management and treatment. Our findings might help physicians for proper selection of antibiotics for treatment of neonatal sepsis.
Neonatal Sepsis,Antibiotics,Antibiogram,resistance
https://ijp.iranpath.org/article_27992.html
https://ijp.iranpath.org/article_27992_b0c10a9aa28da03400847aba022ed31b.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Dose-Dependent Effects of Common Antibiotics Used to Treat Staphylococcus aureus on Biofilm Formation
362
370
EN
Ali
Majidpour
Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
alimajidpour@yahoo.com
Sara
Fathi Zadeh
Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
sara.fathizadeh@yahoo.com
Mastaneh
Afshar
Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
mataneha@yahoo.com
Mohammad
Rahbar
1. Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
2. Department of Microbiology, Reference Health Laboratories Research Centre, Ministry of
Health and Medical Education, Tehran, Iran
rahbar.reflab@gmail.com
Mina
Boustanshenas
0000-0002-5447-9403
Antimicrobial Resistance Research Center, Institute of immunology and infectious diseases, Iran University of Medical Sciences, Tehran, Iran
mina_bustanshenas@yahoo.com
Marjan
Heidarzadeh
Department of biology, faculty of food industry and agriculture standard research institute(SRI),Karaj, Iran
marjan_294@yahoo.com
Leila
Arbabi
Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
leilaarbabi@yahoo.com
Somayeh
Soleymanzadeh Moghadam
Antimicrobial Resistance Research Center, Institute of immunology and infectious diseases, Iran University of Medical Sciences, Tehran, Iran
10.30699/ijp.2017.27993
<strong><em>Background & Objective</em></strong><strong>:</strong><em> Staphylococcus aureus, </em>especially methicillin-resistant <em>S. aureus</em> (MRSA), represent serious nosocomial and community infections. Biofilm formation as an important virulence factor may be affected by sub-inhibitory levels of antibiotics. Few studies examined the effects of all therapeutic antimicrobial agents on clinical <em>S.aureus. </em>The current study aimed at observing the inducing and reducing effects of antibiotics, commonly used to treat staphylococcal infections on the production of staphylococcal biofilm.<br /> <strong><em>Methods</em></strong><strong>:</strong> Four MRSA (1ATCC and 3 clinical) and 1 methicillin-susceptible <em>Staphylococcus aureus </em>(MSSA) strains with biofilm forming ability, evaluated by the Congo red agar (CRA) plate test, were employed. Biofilm formation was measured by crystal violet microtiter plate assay. Cefazolin, rifampicin, vancomycin, oxacillin, clindamycin, cotrimoxazole, minocycline, linezolid, azithromycin, and clarithromycin were added to wells ranging from 0.06to 128 µg/mL (1× to 1/1024 MIC dependent on the MIC value of each strain).<br /> <strong><em>Results:</em></strong> The current study showed that azithromycin and vancomycin had a significant inducing effect on biofilm formation. In contrast, linezolid, cefazolin, and clarithromycin, and in the second place, clindamycin and minocycline could inhibit the level of biofilm production in the sub-minimal inhibitory concentrations.<br /> <strong><em>Conclusion:</em></strong> The findings demonstrated that the biofilm formation as an important virulence factor may be affected by the subinhibitory levels of antibiotics.
Antibiotics,Staphylococcus aureus,Biofilms,Minimum Inhibitory Concentrations,Microplate assays,Therapeutic Uses
https://ijp.iranpath.org/article_27993.html
https://ijp.iranpath.org/article_27993_ab19e74054c35d877682f93b28cf4e8c.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
A Comparative Evaluation of ELISA, PCR, and Serum Agglutination Tests For Diagnosis of Brucella Using Human Serum
371
376
EN
Khashayar
Mohseni
Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
Reza
Mirnejad
0000-0002-7297-3085
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
rmirnejad@yahoo.com
Vahab
Piranfar
Microbiology Department, Islamic Azad University of Tonekabon, Tonekabon, Iran
vahabp@gmail.com
Shiva
Mirkalantari
Microbiology Department, Semnan University of Medical Sciences, Semnan, Iran
sh_mirkalantari@yahoo.com
10.30699/ijp.2017.28293
<strong><em>Background & Objective</em></strong><em>: </em>Since the symptoms of Brucellosis are often atypical and nonspecific, using clinical signs alone to diagnose brucellosis is not advised; therefore, the diagnosis relies predominantly on laboratory testing. Currently, molecular, serological, and microbiological methods are used for diagnosis of this disease. In this study we examined ELISA, PCR and serum agglutination (SAT) methods on human patient serum samples.<br /> <strong><em>Methods:</em></strong>A total of 100 serum samples were collected from suspected patients. Fifty serum samples gave a positive result with the Wright test. The ELISA method was first employed on all samples for the detection of IgG and IgM antibodies against <em>Brucella</em>. Subsequently, the rapid PCR methodology was used to identify presence of <em>Brucella</em> genome in 500 µL of each serum sample. The B4/B5 primer pair was used for PCR amplification.<br /> <strong><em>Results:</em></strong>Out of the 100 serum samples obtained from patients with suspected brucellosis, 50 samples tested positive by SAT and displayed high titers of 1/160. Of these 50 positive samples, 49 samples were positive as per the ELISA test whereas one sample tested negative. The PCR test was conducted on all 100 serum samples and results showed that the 45 serum samples that gave a positive agglutination test were also positive by PCR.<br /> <strong><em>Conclusions</em>: </strong>Various laboratory methods have beenused or introduced for the detection of <em>Brucella</em>. Molecular methods such as PCR, a rapid and sensitive method for detection of bacteria, have also been reported. Based on the results of this study, we propose that the simultaneous use of serology and molecular techniques has the potential to overcome limitations of detection thereby enabling the selection of appropriate treatment for the patient.
Brucella,PCR,ELISA,Agglutination test,human,Serum
https://ijp.iranpath.org/article_28293.html
https://ijp.iranpath.org/article_28293_1000f462dfd346d6075f579a7d7cdc19.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
The Frequency of qnr Genes in Extended-Spectrum β-lactamases and non-ESBLs Klebsiella pneumoniae Species Isolated from Patients in Mashhad, Iran
377
383
EN
Nafiseh
Izadi
Student Research Committee (SRC), Mashhad University of Medical Sciences, Mashhad, Iran.
izadin891@mums.ac.ir
Mahboubeh
Naderi Nasab
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
naderinasabm@mums.ac.ir
Elnaz
Harifi Mood
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
harifie891@mums.ac.ir
Zahra
Meshkat
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
meshkatz@mums.ac.ir
10.30699/ijp.2017.28317
<strong><em>Background and Objectives</em></strong><strong>:</strong> Since the fluoroquinolones are the broad-spectrum antibiotics, they affect both Gram-negative and Gram-positive bacteria. These antibiotics are widely prescribed by physicians. As a result, some bacteria, especially Enterobacteriaceae, have shown a resistance to this family of antibiotics<strong>.</strong> The current study aimed at detecting the frequency of <em>qnrA</em>, <em>qnrB, </em>and <em>qnrS </em>genes, novel plasmid-mediated quinolone-resistance genes, among extended-spectrum β-lactamases (ESBL)-positive and ESBL-negative<em> Klebsiella pneumoniae</em> isolates.<br /> <strong><em>Materials and Methods:</em></strong><strong> </strong>One hundred and thirty isolates of <em>K. pneumoniae</em> were collected from Imam Reza Hospital and its associated clinics from May 2011 to July 2012. The isolates were tested for ESBLs by the conventional methods. Polymerase chain reaction (PCR) was performed to amplify <em>qnr A, B</em>, and <em>S</em>.<br /> <strong><em>Results:</em></strong><strong> </strong>Thirty-eight (29.3%) isolates were ciprofloxacin-resistant. Among 130 <em>K. pneumoniae</em> infectious isolates, 56 (43%) were capable of producing ESBL; 10.8% (n=14), 15.4% (n=20), and 20.8% (n=27) of ESBL-producing <em>K. pneumonia</em> were positive for <em>qnrA, qnrS</em>, and <em>qnrB</em>, respectively, and 13.8% (n=18) of the isolates harbored 2 or 3 <em>qnr</em> genes.<br /> <strong><em>Conclusion:</em> </strong>The results of the current study showed that quinolone-resistance genes were more frequent in ESBL-producing <em>K. pneumoniae</em> (37.5%) isolates, compared with the ESBL-negative isolates (20.89%). The prevalence of <em>qnr</em> genes was high in <em>K. pneumoniae</em> isolates, with higher frequency in ESBL-positive strains. Most of the isolates were positive for all 3 groups of <em>qnr</em> genes and the <em>qnrB</em> was the most common one.
Klebsiella pneumoniae,Quinolones,ESBL (Extended-spectrum B-lactamases),DDS (double disk synergy test)
https://ijp.iranpath.org/article_28317.html
https://ijp.iranpath.org/article_28317_037dc495aef960906216210f79e9107c.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
The Effect of Smoking on Mast Cells Density and Angiogenesis in Chronic Periodontitis
384
391
EN
Noushin
Jalayer naderi
0000-0002-7356-3205
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University
noushin_jly85@yahoo.com
Hasan
Semyari
Department of Periodontic, Faculty of Dentistry, Shahed University
h.semyari@gmail.com
Reza
Hemmati
Faculty of Dentistry, Shahed University
rezahemmati71@yahoo.com
10.30699/ijp.2017.28318
<strong><em>Background and objective:</em></strong>Gingival bleeding reduction in smokers has been associated with decreased blood vessel density. The mechanism of suppressive effect of cigarette smoking on blood vessel density is not precisely defined. The aim of this study was to evaluate the impact of smoking on angiogenesis by assessing mast cells density and VEGF expression in chronic periodontitis.<br /> <strong><em>Materials& Methods:</em></strong> 52 paraffin embedded block of gingiva tissues with periodontitis obtained from 30 nonsmokers and 22 smokers undergoing flap surgery were examined immunohistochemically for VEGF expression. Mast cell counts was completed on toluidine blue stained slides. Exposure to cigarette smoking was calculated by the number of packs × year. Patients were classified into 4 groups based on the number of smoked cigarettes. The correlation between VEGF expression and mast cell counts was evaluated and compared in nonsmokers and smokers. <br /> <strong><em>Results:</em></strong> The mean number of mast cells (p=0.004) and average value of VEGF expression (p = 0.000) in nonsmokers was significantly higher than smokers. No correlation was noted between VEGF expression / mast cell counts and number of smoked cigarettes in four groups of smokers (p=0.29,0.12 , 0.20 and 0.11, respectively).<br /> <strong><em>Conclusion:</em></strong> Mast cells and VEGF expression may account for suppressive effect of cigarette smoking on blood vessels in periodontitis.
Angiogenesis,Chronic periodontitis,Mast cell density,Cigarette smoking,Vascular Endothelial Growth Factor (VEGF)
https://ijp.iranpath.org/article_28318.html
https://ijp.iranpath.org/article_28318_ad2beed2ee5504abb811d60eec159d4f.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
blaVIM and blaIMP Genes Detection in Isolates of Carbapenem Resistant P. aeruginosa of Hospitalized Patients in Two Hospitals in Iran
392
396
EN
Behrang
Kazeminezhad
Department of Pathology, Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
dkazeminezhad@gmail.com
Arezoo
Bostanmanesh Rad
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
a_bmr@yahoo.com
Atoosa
Gharib
Shahid Modarres Clinical research and Development center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
gharib.atoosa@gmail.com
Sara
Zahedifard
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
sara_zfm@yahoo.com
10.30699/ijp.2017.28323
<strong><em>Background & objective</em></strong><em>:</em> Beta-lactam antibiotics resistance specifically Imipenem and Meropenem, the last choices of treatment, causes fatal events in patients with <em>P.aeruginosa</em> infection. The aim of this study was to detect the VIM and IMP of metallo-beta-lactamase genes in 103 isolates of <em>P. aeruginosa </em>in two Iranian hospitals.<br /> <strong><em>Methods</em></strong><strong><em>: </em></strong>In this study, we evaluated the susceptibility of <em>P. aeruginosa</em> to a range of β-lactam antibiotics using disk diffusion method as a standard biochemical test. Combined disk test of Imipenem (IMP) and Imipenem plus Ethylenediaminetetraacetic acid (EDTA) was performed as a phenotypic method to find metallo-beta-lactamase producing isolates.Using conventional PCR method; we evaluated VIM and IMP of metallo-beta-lactamase (MBL) genes in 103 isolates of <em>P.aeruginosa</em>.<br /> <strong><em>Results</em></strong><strong><em>:</em></strong> Twenty six (25.2%) out of 103 isolates were resistant to Imipenem and 26 (25.2%) to Meropenem. Among 26 Imipenem and Meropenem-resistant strains (25.2%), 19 cases (73.0%) were MBL producing. Using PCR method, we detected the bla<sub>VIM </sub>and bla<sub>IMP </sub>genes in 6 (5.8%) and 2(1.9%) of 19 MBL producing isolates, respectively. <br /> <strong><em>Conclusions: </em></strong>Evaluation of these carbepenemases genes improve epidemiologic researches and also, can be used as a diagnostic tool for discriminating between antibiotics resistant and sensitive strains of <em>P.aeruginosa</em> as well as follow-up the patients after treatment.
IMP,VIM,Metallo-β-Lactamase,Pseudomonas aeruginosa
https://ijp.iranpath.org/article_28323.html
https://ijp.iranpath.org/article_28323_6482eaf819337999af900ef95f698374.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Giant Peripheral Ossifying Fibroma of the Posterior Mandible-A Rare Case Report
397
401
EN
Vandana
Reddy
Dept. of Oral Pathology and Microbiology, Subharti Dental College & Hospital, Meerut, India
drvandanareddy@rediffmail.com
Kv
Arunkumar
Dept. of Oral & Maxillofacial Surgery, Subharti Dental College & Hospital, Meerut, India
arunkumarkv@yahoo.com
Vijay
Wadhwan
Dept. of Oral Pathology & Microbiology, Subharti Dental College & Hospital, Meerut, India
pathogen@rediffmail.com
Arvind
Venkatesh
Dept. of Oral Pathology & Microbiology, Subharti Dental College & Hospital, Meerut, India
drarvindvenkatesh@gmail.com
10.30699/ijp.2017.28320
Large, atypical peripheral ossifying fibromas are known as giant peripheral ossifying fibromas. These lesions have often been associated with heterogeneous clinical and radiographic characteristics subsequently leading to their misdiagnosis. Biopsies have been the gold standard for the diagnosis of such lesions. This study reports on an acute presentation of giant peripheral ossifying fibroma, clinically mimicking a malignant lesion due to its atypical presentation along with its characteristic histological features, which led to the establishment of the diagnosis.
Giant gingival neoplasms,gingival growths,ossifying fibroma
https://ijp.iranpath.org/article_28320.html
https://ijp.iranpath.org/article_28320_604885aa9bce79241275714f2adc9f20.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Sclerosing Stromal Tumor: A Rare Ovarian Neoplasm
402
405
EN
Shilpa
Bairwa
Senior Resident, Deptt of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, India
dr.shilpabairwa16@gmail.com
Rahul
Satarkar
Associate Professor, Deptt of Pathology, SHKM, GMC, Nalhar,
Mewat, Haryana, India
rsatarkar@gmail.com
Shivani
Kalhan
Professor, Deptt of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, India.
shivani_kalhan@yahoo.co.in
Shilpa
Garg
Assistant Professor, Deptt of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, India.
manojdahakwal25602@gmail.com
Ashok
Sangwaiya
Assistant Professor, Deptt of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, India.
ashoksangwaiya25602@gmail.com
Pawan
Singh
Assistant Professor, Deptt of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, India
bodwalps@gmail.com
10.30699/ijp.2017.28321
Ovarian sex cord-stromal tumors are relatively infrequent neoplasms that account for approximately 8% of all primary ovarian neoplasm. Sex cord-stromal tumors of the ovary include granulosa cell tumors, fibrothecomas, Sertoli-Leydigcell tumors, steroid cell tumors, and sclerosing stromal tumors (SST). Sclerosing stromal tumors account for 2% to 6% of ovarian stromal tumors. Despite the rarity of this particular neoplasm, it is not always possible to predict the presence of this tumor preoperatively on the basis of clinical and sonographic findings. Histopathological and immunohistochemical (IHC) examinations confirm the diagnosis. Herein, the clinical findings and histopathological features of SST are described in a 24-year-old female.
Benign ovarian neoplasm,Pseudolobular pattern,Sclerosing stromal tumor
https://ijp.iranpath.org/article_28321.html
https://ijp.iranpath.org/article_28321_cb161c64b0935a965119a73716394aaf.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Intramuscular Hibernoma of the Scapular Region Misdiagnosed on Cytology as a Malignant Lesion: A Report of a Rare Case
406
409
EN
Mukta
Pujani
0000-0003-3953-1864
Department of Pathology, ESIC Medical College, Faridabad, India
drmuktapujani@gmail.com
Sabina
Khan
Department of Pathology, Hamdard Institute of Medical Sciences & Research, Jamia Hamdard, New Delhi, India
drsabina1@gmail.com
Sujata
Jetley
Department of Pathology, Hamdard Institute of Medical Sciences & Research, Jamia Hamdard, New Delhi, India
drjetley2013@gmail.com
Prabhat
Kumar
Raina
Dr. Prabhat Kumar Raina, MBBS, MS (Surgery)
Previously
Assistant Professor, Department of Surgery,
Hamdard Institute of Medical Sciences and Research,
Jamia Hamdard, New Delhi
drpkraina@gmail.com
Sujata
Jetley
Department of Oncosurgery, RJSP Cancer Hospital, Ranchi, India
nayana104@yahoo.com
10.30699/ijp.2017.28322
Hibernomas are extremely rare benign tumors of adipose tissue characterized by an admixture of brown fat cells with granular, multivacuolated cytoplasm and white fat cells. Hibernomas account for 1.6% of benign adipose tissue tumors and approximately 1.1% of all adipocytic tumors. Around 10% of these cases are intramuscular. It was initially described in the early 1900s as being composed of brown fat. Hibernomas usually occur in third to fourth decades of life and the most frequent sites being thigh, trunk, shoulder, back etc. Cytological differential diagnoses of hibernoma include well differentiated liposarcoma, myxoid/round cell liposarcoma, chondroid lipoma and benign granular cell tumor. Due to its abundant vascularity evident on angiography, it can sometimes mimic a malignant lesion, from which it needs to be distinguished as complete surgical excision is the only treatment required for a hibernoma. Moreover, it has no malignant or metastatic potential.<br /> We presented a rare case of intramuscular hibernoma of the scapular region in a 34-year-old male, in which cytology was reported as pleomorphic fibrolipomatous malignant lesion.
Hibernoma,benign lipomatous tumor,brown fat,intramuscular hibernoma,scapular
https://ijp.iranpath.org/article_28322.html
https://ijp.iranpath.org/article_28322_b353cc25e87f1b0df12f179026b30581.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
Nodular Cystic Basal Cell Carcinoma of the Trunk: a Diagnostic Dilemma in an Unsuspecting Youth
410
412
EN
Ruchita
Tyagi
Department of Pathology, Dayanand Medical College & Hospital, Tagore Nagar
ruchitatyagi@gmail.com
Dilpreet
Kaur
Department of Pathology,
Dayanand Medical College & Hospital,
Tagore Nagar,
Ludhiana, Punjab,
India
dilpreetrandhawa@ymail.com
Gursheen
Kaur
Senior Resident Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP
gursheengs@sify.com
Bhavna
Garg
Department of Pathology, Dayanand Medical College & Hospital, Tagore Nagar, Ludhiana, Punjab, India
dr.bhavnagarg@yahoo.co.in
Neena
Sood
Professor & Head, Department of Pathology, Dayanand Medical College & Hospital, Tagore Nagar, Ludhiana, Punjab, India
drneena_sood@yahoo.co.in
Sunil
Gupta
Professor & Head, Department of Skin & Venereal diseases, Dayanand Medical College & Hospital, Tagore Nagar, Ludhiana, Punjab, India
vsunilgupta@rediffmail.com
10.30699/ijp.2017.28319
Basal cell carcinoma (BCC) commonly affects the elderly and is mostly confined to the head and neck region. Only 10% of all cases occur on the trunk. We presented a case of bullous lesion on the abdomen in a young male, initially diagnosed by clinicians as a vascular nevus. Histopathological examination and immunohistochemistry (IHC) revealed it to be Nodular cystic variant of BCC. This rare variant of BCC morphologically resembles benign skin adnexal tumor of Eccrine syringofibroadenoma. Ber Ep4 positivity on IHC established the correct diagnosis. This case highlights that nodular cystic variant of BCC can be a diagnostic dilemma for clinicians and pathologists.
Basal cell carcinoma,cystic,Nodular,trunk
https://ijp.iranpath.org/article_28319.html
https://ijp.iranpath.org/article_28319_4746f147a5f8e4dd1e454ff48701f214.pdf
Farname Inc in collaboration with Iranian Society of Pathology
Iranian Journal of Pathology
1735-5303
2345-3656
12
4
2017
12
01
A Simplified Working Classification Proposed for Myxoid Tumors of Oral Cavity
413
414
EN
Manas
Bajpai
Department of Oral and Maxillofacial Pathology, NIMS Dental College, Jaipur, India
dr.manasbajpai@gmail.com
Nilesh
Pardhe
Department of Oral and Maxillofacial Pathology, NIMS Dental College, Jaipur, India
drpardhenilesh@gmail.com
10.30699/ijp.2017.28324
Dear Editor-in-Chief<br /> Myxoid tumors of oral cavity encompass a broad spectrum of lesions, primarily neoplastic.(1) The significant variations in the biological behaviour; ranging from completely harmless to malignant neoplasm they require an accurate histopathological diagnosis to ensure a proper treatment. (2) Because of the considerable overlapping between the clinical and histopathological features, this group of tumors often produce a diagnostic difficulty to the clinicians and oral pathologists.(3) An exhaustive review of the literature reveals that there is no approved classification of myxoid tumors of oral cavity. A simple working classification of myxoid tumors is proposed here.
Myxoid tumors,oral cavity,working classification
https://ijp.iranpath.org/article_28324.html
https://ijp.iranpath.org/article_28324_f0a41e9172e64054517e2a378df4b263.pdf