%0 Journal Article %T An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA %J Iranian Journal of Pathology %I Farname Inc in collaboration with Iranian Society of Pathology %Z 1735-5303 %A Salehi, R, %A Tabanifar, B. %A Asgarani, E. %A Faghihi, M. %A Allame, T. %D 2008 %\ 09/01/2008 %V 3 %N 4 %P 173-178 %! An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA %K DNA %K Paraffin Embedding %K Polymerase chain reaction %R %X   Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation. Results: This method produced DNA suitable for amplification using primers specific for human SMN and β globin genes in 98.63% and 82.2% of samples respectively. We also detected human papillomavirus DNA in 58.33% of appropriate samples. Conclusion: This procedure provides simple and efficient method for recovery of amplifiable genomic and viral DNA from paraffin wax embedded tissues.   %U https://ijp.iranpath.org/article_9002_917d8daf6ef67bc61d30f62c5c3ea8bc.pdf