@article { author = {Ahangar-Parvin, Rayhaneh and Mohammadi-Kordkhayli, Marzieyeh and Azizi, Sayyed Vahab and Nemati, Maryam and Khorramdel-Azad, Hossian and Taghipour, Zahra and Hassan, Zuhair and Moazzeni, Seyed Mohammad and Jafarzadeh, Abdollah}, title = {The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis}, journal = {Iranian Journal of Pathology}, volume = {13}, number = {1}, pages = {10-22}, year = {2018}, publisher = {Farname Inc in collaboration with Iranian Society of Pathology}, issn = {1735-5303}, eissn = {2345-3656}, doi = {10.30699/ijp.13.1.10}, abstract = {Background & objective The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE). Methods: EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively. Results: IL-12 gene expression and its serum levels in PBS-injected- or OO-administrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected- or OO-administrated EAE groups (P Conclusion: VD modulates the expression of IL-12 and TGF-β in spinal cord and serum of EAE mice.}, keywords = {Experimental Autoimmune,Encephalomyelitis,Vitamin D,IL-12,TGF-β}, url = {https://ijp.iranpath.org/article_29937.html}, eprint = {https://ijp.iranpath.org/article_29937_a54c4d3157b9545cdcc5c422b94a3f23.pdf} }