Hematopathology
Soroush Felezi; Anahita Nosrati; Mohammad Eslami Jouybari; Javane Jafarshad
Abstract
Dendritic cells (DCs) are key arms of immune system, which act in antigen presenting processes, and are considered as a bridge between innate and adaptive immune responses. DCs are found in both lymphoid and non-lymphoid organs. They are called interdigitating dendritic cells (IDCs) in secondary lymphoid ...
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Dendritic cells (DCs) are key arms of immune system, which act in antigen presenting processes, and are considered as a bridge between innate and adaptive immune responses. DCs are found in both lymphoid and non-lymphoid organs. They are called interdigitating dendritic cells (IDCs) in secondary lymphoid organs. IDCs lack lineage surface markers and are positive for S-100 and vimentin. IDC sarcoma (IDCS) is a very rare neoplasm, which mainly affects lymph nodes, though there are reports of extra-nodal involvement. IDCS is thought to have poor prognosis. Although there is no consensus on the treatment modalities, such options as radicalsurgery, chemotherapy, and radiotherapy are performed depending on severity and site of the lesion. In this study, we present a case of IDCS in a 53-year-old male with a history of several skin lesions and prior diagnoses of basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and metatypical carcinoma (MTC).
Shahriar Dabiri; Hadiseh Zakeridana; Manzoumeh Shamsi Meymandi; Abbass Etminan; Jalal Azmandian; Simindokhet Habibzadeh; Farzaneh Khazanfari; Saeideh Parvaresh
Volume 8, Issue 4 , October 2013, , Pages 255-262
Abstract
Background & Objectives: Chronicity of lupus nephritis (LN) should be considered for interaction of cell mediated immunity (CMI) and dendritic cells in glomeruli and tubulointerstitial areas. In this study establishment of immunohistopathological changes of dendritic cells and other immune effector ...
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Background & Objectives: Chronicity of lupus nephritis (LN) should be considered for interaction of cell mediated immunity (CMI) and dendritic cells in glomeruli and tubulointerstitial areas. In this study establishment of immunohistopathological changes of dendritic cells and other immune effector cells in lupus nephritis comparing with non-lupus nephritis was performed.
Materials & Methods: Renal needle biopsies of 35 cases of lupus nephritis and 35 cases of other causes of persistent proteinuria were compared for immunohistochemistry for plasmacytoid (CD123), myeloid (CD11c) dendritic cells, macrophages (CD68) and lymphocytes (CD4) markers. Statistical analysis of the data was performed using Spearman and Pearson correlation or ANOVA and t- student test (P < 0.05).
Results:Significant difference of glomerular and interstitial spaces for presence of myeloid-plasmacytoid dendritic cells and lymphocytes except macrophages between lupus nephritis and other causes of persistent proteinuria have found (P<0.001). Positive significant correlations were observed between glomerular presentation of myeloid dendritic cells and chronicity index but not with other markers in lupus nephritis (P <0.001). Statistically significant changes between presence of all markers and activity index were not observed (P >0.05).
Conclusions: The myeloid dendritic cells might have synergistic role with other immune cells in pathogenesis and progression or chronicity of lupus nephritis.
Majid Sadeghizadeh; Abbas Nikravesh; Mehrdad Behmanesh; Liam Good
Volume 4, Issue 1 , January 2009, , Pages 13-18
Abstract
Background & Objectives: Antisense peptide nucleic acids (PNA) that target growth essential genes show potent bactericidal properties without cell lysis. We considered the possibility that whether PNA treatment influence the bacteria total nucleic acids content and apply approach to develop ...
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Background & Objectives: Antisense peptide nucleic acids (PNA) that target growth essential genes show potent bactericidal properties without cell lysis. We considered the possibility that whether PNA treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to Dendritic cells (DCs). DCs are the most potent antigen presenting cells in the immune systems. Since the uptake of bacteria by DC is a necessary step for generation of effective DNA vaccine, we studied the uptake efficiency of PNA treated bacteria by DCs. Material & Methods: Total nucleic acids of anti- acyl carrier protein (acpP) peptide-PNA treated Escherichia coli Hb101 containing plasmid have been isolated. In addition, peripheral blood monocytes have been purified using mouse anti- cluster of differentiated (CD14) coated magnetic beads and then culture in presence of growth factors. Generated DCs have been assessed for their ability of uptake of flurescein isothiocyanate (FITC) - labeled peptide-PNA treated bacteria and heat inactivated by flow cytometry. Results: The preparation of total nucleic acid from peptide-PNA treated showed five distinct bands which correspond to chromosomal DNA, plasmid DNA, 23S ribosomal RNA (rRNA), 16S rRNA and mixture of small RNA. DCs incubated with FITC- labeled peptide-PNA treated and heat inactivated bacteria showed almost equal fluoresence intensity. Conclusion: Peptide–PNA treatment bacteria are intact and do not appear to alter cell barriers and nucleic acid content. They can be taken up by DCs efficiently.These finding may confirm new application for peptide-PNA in immunology and DNA vaccine.