Pulmonary Pathology
Sotoudeh Mohammadi; Mitra Rezaei; Fatemeh Shojaeian; Mihan Pourabdollah; Leila Mohammadi Ziazi; Sharareh Seifi; Atousa Doroodinia; Babak Salimi; Adnan Khosravi; Mohammad Amin Farhangnasab
Abstract
Background & Objective: Various studies showed the use of epidermal growth factor receptors (EGFRs) gene mutations in the therapeutic plan of patients with advanced lung cancer. This study aimed to investigate the frequency and types of EGFR gene mutations among Iranian patients with lung adenocarcinoma ...
Read More
Background & Objective: Various studies showed the use of epidermal growth factor receptors (EGFRs) gene mutations in the therapeutic plan of patients with advanced lung cancer. This study aimed to investigate the frequency and types of EGFR gene mutations among Iranian patients with lung adenocarcinoma referred to a specialized lung diseases hospital from 2014 to 2019.Methods: The data of all patients with lung adenocarcinoma referred to the Molecular Department of Masih Daneshvari Hospital Laboratory (National Research Institute of Tuberculosis and Lung Diseases) from 2014 to 2019 for EGFR mutation tests were collected. Patients' characteristics data and information on the frequency and types of EGFR gene mutations were obtained from the hospital information system (HIS). The collected data were analyzed using SPSS 25.Results: A total of 570 individuals (Mean age of 58.74, 51.6% Male) were included in the study; 113 out of 570 patients (19.8%) were diagnosed with gene mutation. In terms of the type of mutation, 65 participants (57%) showed deletion, 48 patients (42.1%) were diagnosed with replacement, and one (0.9%) case demonstrated both. Notably, the mutation rate detected among the female patients was significantly higher than the male ones (P=0.001); in particular, deletion type of mutation was found more among women, although both genders were the same in terms of the replacement frequency. However, the age had no effect on the mutation in this study (P=0.05).Conclusion: Among Iranian patients with lung adenocarcinoma, 19.8% harbored EGFR gene mutation. This mutation was found in association with lung cancer and could affect the patient's therapeutic plan.
Nephropathology
Shirin Taraz Jamshidi; Khadijeh Sajjadian; Maryam Emadzadeh; Malihe Saber Afsharian; Mahmoud Reza Kalantari; Anita Alenabi; Abbas Ali Zeraati; Ali Emadzadeh
Abstract
Background & Objectives: Polyomavirus-associated nephropathy (PVAN), mainly caused by the BK virus, is one of the most important infectious complications of kidney transplantation. The leading histopathologic characteristics of PVAN is viral cytopathic effects, such as nucleomegaly with smudged or ...
Read More
Background & Objectives: Polyomavirus-associated nephropathy (PVAN), mainly caused by the BK virus, is one of the most important infectious complications of kidney transplantation. The leading histopathologic characteristics of PVAN is viral cytopathic effects, such as nucleomegaly with smudged or clumped chromatin and intranuclear ground-glass inclusion, mostly in tubular epithelial cells. Moreover, tubular necrosis, tubulitis, interstitial inflammation, atrophy, and fibrosis have been noted. Positive immunohistochemistry (IHC) staining for SV-40 highlights the infected epithelial cells of renal tubules. Methods: A total of 85 core needle biopsies of transplanted kidneys were evaluated histologically and were stained for SV-40 using the IHC method. In addition, a follow-up of graft failure was performed. Results: Our findings revealed that the frequency of polyomavirus infection in kidney transplant patients in the Northeast of Iran is 4.7%. There was no significant correlation between PVAN and graft rejection. Although a higher rate of graft loss was observed in PVAN patients, in comparison with non-PVAN patients (25% vs. 14.8%), the difference was not statistically significant. Moreover, patients with immunohistochemically confirmed PVAN and those with histopathologic features of viral-like cytopathic effects had significantly lower graft survival in the follow-up period (42.5 vs. 196.8 months and 109.4 vs. 205.7 months, respectively). Conclusion: The frequency of polyomavirus infection in kidney transplant patients in the Northeast of Iran is 4.7%. There was no significant correlation between PVAN and graft rejection. Furthermore, we observed that polyomavirus infection accelerates the course of graft loss.
Hedieh Moradi Tabriz; Alireza Abdollahi; Saeideh Mahfoozi
Volume 5, Issue 3 , June 2010, , Pages 143-149
Abstract
Background and Objectives: Blood stream infections cause significant morbidity and mortality in the world. In this study, we aimed at describing the organisms responsible for septicemia in 2006-2007 and their antimicrobial susceptibility that might lead to proper selection of antimicrobial agents ...
Read More
Background and Objectives: Blood stream infections cause significant morbidity and mortality in the world. In this study, we aimed at describing the organisms responsible for septicemia in 2006-2007 and their antimicrobial susceptibility that might lead to proper selection of antimicrobial agents in hospitalized patients with suspected sepsis. Materials and Methods: Microbiology reports of 1753 blood specimens collected from inpatients of a referral hospital in Iran were retrospectively reviewed. Specimen culture, bacterial identification, and disk diffusion susceptibility testing were performed according to The Clinical and Laboratory Standards Institute guidelines. Data were analyzed by SPSS, X² Test and the fisher exact Test. Results: Coagulase-negative Staphylococci accounted for most isolated bacteria (24.61%), in both genders. The second and third most frequent isolated bacteria in adults were staphylococcus aureus and Stenotrophomonas maltophilia, and in children were Klebsiella pneumoniae and S. aureus. Among the all Gram – positive cocci, vancomycin was the most effective agent. The most effective antibiotic varied among the Gram-Negative isolates, for example 95.65% of S. maltophilia were susceptible to co-Trimoxazole, and amikacin were suitable antibiotic in 85.29% of E. coli. Conclusion: As the degree of antibiotic resistance rate for blood stream pathogens is alarming, it is mandatory to monitor the susceptibility of these isolates in order to avoid inappropriate use of antibiotics in hospital wards
