Nasser Rakhshani; Mohammadreza Araste; Farid Imanzade; Mahshid Panahi; Fahimeh Safarnezhad Tameshkel; Masoud Reza Sohrabi; Mohammad Hadi Karbalaie Niya; Farhad Zamani
Volume 11, Issue 4 , October 2016, , Pages 409-415
Abstract
Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic ...
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Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections from suction rectal biopsies. Calretinin immunohistochemistry (IHC) may be a useful in its diagnosis. This study aimed to proof the usefulness of immunohistochemical staining for calretinin in rule out of Hirschsprung disease. Methods: Paraffin blocks and slides were retrieved from the pathology archives of Ali Asghar Hospital, Tehran, Iran from 2007 to 2011 with pathology report based on the presence (14 patients) or absence (70 patients) of ganglion cells and transitional zone anatomical region (10 patients). Slides were stained with hematoxylin and eosin method to confirm the initial diagnosis was verification again. After preparing the slides, they were stained by IHC for calretinin. Then, the results were analyzed using SPSS software. Results: In most patients, IHC for calretinin provided highly compatible results with hematoxylin-eosin findings in diagnosis of Hirschsprung disease. The values of specificity and accuracy between calretinin and standard histology (H&E) compared by the Fisher exact test declared calretinin presented significantly higher specificity and accuracy values than H&E staining (P <0.0001). Conclusion: Calretinin IHC overcomes most of the difficulties encountered using the histology hematoxylin-eosin. Then, IHC for calretinin is a good ancillary method used by pathologists in diagnosis of Hirschsprung disease.
Zohreh Yousefi; Nourieh Sharifi; Farnoosh Sadatmand; Soodabeh Shaid Salles
Volume 4, Issue 4 , September 2009, , Pages 172-176
Abstract
Background and Objective: Histopathological evaluation of granulosa cell tumors (GCT) of the ovary may be confused morphologically with a wide variety of the tumors. Immunohistochemical staining for inhibin and calretinin can be used for better diagnosis. Although it has been suggested that inhibin can ...
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Background and Objective: Histopathological evaluation of granulosa cell tumors (GCT) of the ovary may be confused morphologically with a wide variety of the tumors. Immunohistochemical staining for inhibin and calretinin can be used for better diagnosis. Although it has been suggested that inhibin can be a sensitive marker for GCT, it maybe had negative results in some cases. In addition, caltrinin has been proposed as a marker for GCT. The aim of this study was to investigate the immunohistochemical methods (IHC) including a comparison of calretinin and inhibin markers in the diagnosis of these tumors. Patients and Methods: This prospective study carried out from 2000 to 2009 at Ghaem and Omid hospitals, Mashad University of Medical Sciences, Iran. A total of 23 ovarian GCT specimens were immunostained with commercially available antibodies to find out calretinin and inhibin immunoreactivity. Data were analyzed by descriptive statistical method. A P value of =Results: For diagnosing GCT, the sensitivity of calretinin was 100% and that of the inhibin was almost 73.9%. The extent and severity of staining was more extensive for calretinin compared to inhibin PConclusion: Calretinin is a more sensitive biomarker for GCT than inhibin.