Ghodsie Alavi; Nourieh Sharifi; Ali Sadeghian; Alireza Rezaei; Hossein Shidaee
Volume 7, Issue 3 , July 2012, , Pages 151-156
Abstract
Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, ...
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Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, some studies did not detect HPV DNA in ovarian carcinoma tissues. In this article, we investigated the potential role of high risk HPVs in the ovarian epithelial carcinoma.
Methods: Fifty archived epithelial ovarian cancer paraffin blocks were collected. Then, 30 non-malignant ovarian blocks used as control. These samples were histopathologically were confirmed by a pathologist and the proper blocks for DNA extraction and PCR were sorted. PCR was conducted deploying highly specific primers for high-risk types of HPV (18 and 16) according to the instructions of manufacturer company.
Results: High-risk oncogenic sequences were identified in 4 (5%) of the 80 studied samples. Of the 4 HPV positive cases, there was 1 case with normal tissue, 1 case of mucinous cyst adenocarcinoma, and 2 cases of serous cyst adenocarcinoma
Conclusion: Surprisingly, our findings could not support any association between high-risk oncogenic human papilloma virus (18 and 16) and malignant ovarian epithelial cancer. Therefore, that HPV is highly unlikely to play any causal role in the pathogenesis of epithelial ovarian neoplasia.
Zahra Meshkat; Hessam Mirshahabi; Hoorieh Soleimanjahi; Zuhair Mohamad Hassan
Volume 4, Issue 2 , April 2009, , Pages 65-70
Abstract
Background and Objectives: Some of the human papillomaviruses (HPVs) can infect genital tracts and are sometimes associated with anogenital tract cancers. HPVs induced cervical cancers through the expression of E6 and E7 genes by inactivating the tumor suppressor proteins. In this study, E6 and ...
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Background and Objectives: Some of the human papillomaviruses (HPVs) can infect genital tracts and are sometimes associated with anogenital tract cancers. HPVs induced cervical cancers through the expression of E6 and E7 genes by inactivating the tumor suppressor proteins. In this study, E6 and E7 genes were chosen in order to construct an expression vector which is able to express target proteins. Patients and Methods: This experimental investigation was performed in Virology Department of Tarbiat Modares University. An expression vectorcontaining human papillomavirus type 16 E6 and E7 genes was constructed. The accuracy of the plasmid was confirmed by polymerase chain reaction (PCR) and restriction enzyme analysis. The construct was transfected into the eukaryotic cells and its ability for protein production was confirmed by Western blotting. Results:The colonies containing desired plasmid have the fragment about 995 bp. For confirming the ability of the construct for protein production in eukaryotic cells, Western blotting was done using the lyses-cells as antigen and they showed the desired bands using monoclonal antibodies. Conclusion: The designed vector can consider as a based vaccine for construction a therapeutic vaccine in suitable vectors for gene therapy in order to administration in Iranian patients with cervical cancer.