Shahriar Dabiri; Hassan Manafi Anari; Simin Shamsi Meymandi; Reza Fotouhi Ardakani; Sahar Amirpour Rostami; Manzumeh Shamsi Meymandi; Perikala Vijayananda Kumar
Volume 8, Issue 4 , October 2013, , Pages 247-254
Abstract
Background and Objectives: Kerman Province, especially city of Bam in the southeast part of Iran, is epidemics for dry type cutaneous leishmaniasis (DTCL). This study was conducted to compare the effect of different treatments on parasite DNA load following therapies using Real-Time PCR method.
Materials ...
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Background and Objectives: Kerman Province, especially city of Bam in the southeast part of Iran, is epidemics for dry type cutaneous leishmaniasis (DTCL). This study was conducted to compare the effect of different treatments on parasite DNA load following therapies using Real-Time PCR method.
Materials and Methods: Fifteen patients were divided into three groups under therapy with intralesional meglumine antimoniate, topical imiquimod and combination of both drugs. After obtaining consent from patients, punch biopsies were taken before and after treatment .To compare the amount of DNA load a relative quantitative Real-Time PCR method was designed and set up using Leishmania tropica ITS (internal transcribed spacer) gene. After doing PCR, the obtained results were analyzed using 2^-ΔΔCT method and relativity of DNA load before and after treatment were calculated.
Results: The highest falling of DNA load was for imiquimod (mean 4/7 cases), glucantime (mean 2/2 cases) and combination therapy (mean 2/4). From clinical point of view combination therapy had the best response. On the other hand, the overall IHC findings showed good response based on decreased CD1a epidermal, increased CD1a dermal, decreased CD68 macrophages and increased CD3 and CD20 of dermis.
Conclusion: We set up a new method to compare Leishmania DNA load using the stable human gene of beta actin for normalization. We concluded that imiquimod was immune modulator and had synergistic effects on the best parasitocidal drug of glucantime for better response.
Hamid Tabrizchi; Shahriar Dabiri; Alireza Soutodehnejad; Bahram Azadeh; Malcolm M.M. Hayes
Volume 2, Issue 1 , January 2007, , Pages 41-44
Abstract
Localized Leishmania Lymphadenitis (L.L.L) is a self limited clinical presentation of cutaneous leishmaniasis. Microscopic findings in L.L.L are very similar to Toxoplasma lymphasenitis. In all cases, an intensive microscopic search should be done for Leishman bodies. In this study, we describe our microscopic ...
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Localized Leishmania Lymphadenitis (L.L.L) is a self limited clinical presentation of cutaneous leishmaniasis. Microscopic findings in L.L.L are very similar to Toxoplasma lymphasenitis. In all cases, an intensive microscopic search should be done for Leishman bodies. In this study, we describe our microscopic findings and results of our immnohistochemical (IHC) study with a panel of monoclonal antibodies against T-Cells, B-Cells, histiocytes, Langerhans cells, and dendritic reticulum cells. Morphologic and immunohistochemical study in this case showed expanded paracortical tissue with many small clusters of epithelioid histiocytes and large reactive B follicles. Immature sinus histiocytosis in subcapsular and trabecular sinuses was also seen.