Alireza Abdollahi; Issa Jahanzad; Siamak Fakhrian
Volume 3, Issue 2 , March 2008, , Pages 55-60
Abstract
Background and Objective: Glumerular diseases are among the most prevalent causes of renal chronic insufficiencies. This study aimed to analyze the prevalence of various kinds of glumerulonephritis based on the findings of electron microscope. Materials and Methods: This study had a descriptive ...
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Background and Objective: Glumerular diseases are among the most prevalent causes of renal chronic insufficiencies. This study aimed to analyze the prevalence of various kinds of glumerulonephritis based on the findings of electron microscope. Materials and Methods: This study had a descriptive retrospective and cross sectional strategy. Slides of patients (124 cases) who had undergone kidney biopsy during a one-year period due to renal diseases were reviewed and compared. The required data were collected and analyzed using SPSS software. Results: It was found out that 52.4% of the patients were female and 47.6% were male. The average age of the female patients was 28.26 and of the male ones was 29.8 years old. The most prevalent type of glumerulonephritis was membranous and the most prevalent stage was stage II. The prevalent fluorescence pattern of the IgG deposits in the basal membrane of glomerulus. Conclusion: Regarding the variety in the prevalence of different kinds of glumerulonephritis fond within different ages and sex, all cases should be taken into consideration while dealing with the patients. It should also be noticed that immunofluorscence is a complementary diagnosis method and does not have much use without electron and light microscope. In those cases where several contradictory diagnoses have been suggested in light microscope or a particular change has not been observed or the sample has not been sufficient to be analyzed, electron microscope has a final and significant role.
Alireza Abdollahi; Issa Jahanzad; Peyman Mansuri Mehrabani
Volume 3, Issue 2 , March 2008, , Pages 88-94
Abstract
Background and Objective: In this study, we explored expression rate, some biomarkers affecting the prognosis of the breast carcinoma, and the relationship between these markers and clinicopathologic features of the disease as well as the relationship between each of these markers through a tissue ...
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Background and Objective: In this study, we explored expression rate, some biomarkers affecting the prognosis of the breast carcinoma, and the relationship between these markers and clinicopathologic features of the disease as well as the relationship between each of these markers through a tissue array technique. Materials and Methods: This study was an observational and cross-sectional study. From 100 breast samples which had been diagnosed as invasive ductal carcinoma, blocks were prepared through a tissue array method and were stained by monoclonal antibodies of the markers. All data were analyzed using SPSS program. Results: The appearance rate of EGF-R marker had a direct relationship with the degree of malignancy (p=0.026), metallothionein marker with the mean number of mitosis (p=0.044), sialyl-Tn marker with the macroscopic size of tumor (p=0.036), the appearance of cyclin B1 marker with the appearance of metallothionein marker (p=0.012), and the appearance rate of EGF-R marker had a reverse relationship with Nm23 (p=0.020). Conclusion: Through investigating the relationship between some biomarkers such as EGF-R and metallothionein and the clinicopathogenic features of tumor or the relationship between each marker and the other parameters, we can assess the state of invasion and metastasis process or the degree of its malignancy or determine its prognosis.
Issa Jahanzad; Amir Hossein Sadrzadehrafie; Reza Rafie; Masoud Rahmanian; Mohammad Reza Hayeri; Ali Reza Rezaee; Babak Gharaei; Majid Sadeghizadeh; Ali Mohammad Ahadi
Volume 2, Issue 1 , January 2007, , Pages 17-22
Abstract
Background and Objective: The association of EBV with Hodgkin Lymphoma (HL) has been intensely investigated over the last few years. EBV is also associated with several other malignancies. On the other hand, Ki67 molecule serves as a widely accepted proliferation marker. Several studies were previously ...
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Background and Objective: The association of EBV with Hodgkin Lymphoma (HL) has been intensely investigated over the last few years. EBV is also associated with several other malignancies. On the other hand, Ki67 molecule serves as a widely accepted proliferation marker. Several studies were previously performed about the expression of Ki67 in HD. This study tried to detect the correlation between Ki67 expressions with chronic EBV infection in HD patients of Iran by attenuating confounding factors.
Materials and Methods: Hodgkin patients were divided into two groups regarding their EBV infection status. The case and control groups were matched for the stage of the disease. Immunohistochemical methods were used to detect Ki67 expression while DNA extraction and PCR amplification were performed to indicate chronic EBV infection.
Results: Clinicopathologic criteria of two groups including male to female ratio, age, presence of B symptoms, and pathologic subtypes were not significantly different. Ki67 expressed in 21% of EBV infected cells while 30% of EBV negative cells had this marker. There was also no statistically significant difference between these two groups.
Conclusion: After omitting the possible effect of confounding factors such as the presence of other malignancies and advanced disease stage, there was no correlation between Ki67 expression and EBV infection in Hodgkin patients in this study.
