Arezoo Aghakhani; Ali Eslamifar; Mehdi Razzaghi Abyaneh; Mohsen Vazir-Nezami; Habibollah Moghadasi; Amitis Ramezani; Masoomeh Shams-Ghahfarokhi; Ali Khameneh; Mohammad Ali Shokrgozar; Ali Akbar Amir-Zargar; Zahra Deljoodokht
Volume 3, Issue 3 , June 2008, , Pages 135-149
Abstract
Background and Objective: Chronic rhinosinusitis (CRS) is one of the most common chronic diseases. In the past decades, there has been an increase in fungal infections of sinuses and fungal rhinosinusitis (FRS) has been diagnosed more frequently. Knowing the fungal flora and its prevalence in ...
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Background and Objective: Chronic rhinosinusitis (CRS) is one of the most common chronic diseases. In the past decades, there has been an increase in fungal infections of sinuses and fungal rhinosinusitis (FRS) has been diagnosed more frequently. Knowing the fungal flora and its prevalence in CRS patients will allow a better understanding of this disease, permitting a correct diagnosis, treatment and formulating its prognosis. This study was performed to assess the fungal flora and its prevalence in CRS patients. Materials and Methods: In this prospective study fungal cultures were obtained from nasal and paranasal sinuses mucus of 100 patients suffering from CRS and 40 controls who did not show any evidence of CRS .In addition, in CRS patients, paranasal sinuses mucus and tissue were histologically investigated for evidence of eosinophilic granulocytes and fungal elements besides fungal culture. Results: Fungal cultures of nasal mucus were positive in 62.5% and 60% of cases and controls respectively. Positive fungal cultures of paranasal sinuses mucus were seen in 49% of cases and 5% of controls. Aspergillus was the most frequent, followed by Penicilium and Cladosporium in both nasal and paranasal sinuses mucus culture. Eosinophilic mucin was found in 90% of CRS patients. Conclusion: Our data showed that fungal infection is frequent in patients with chronic rhinosinusitis. Based on our results, Aspergillus was the most frequent isolated fungus in CRS patients.
Ali Eslamifar; Farrokh Tirgari; Rasool Hamkar; Amitis Ramezani; Hossein Frootan pishbigar; Shahrum Mirmomen; Azin Nahvigoo; Vahideh Shahnazi; Zahra Deljoodokht; Shifteh Vahidi; Arezoo Aghakhani
Volume 2, Issue 1 , January 2007, , Pages 11-16
Abstract
Background and Objective: Human papillomavirus (HPV) is one of the possible etiologic factors in development of esophageal squamous cell carcinoma (ESCC). In this study we aimed to study the role of HPV in ESCC.
Patients and Methods:In this study, 140 cases of ESCC were analyzed for the ...
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Background and Objective: Human papillomavirus (HPV) is one of the possible etiologic factors in development of esophageal squamous cell carcinoma (ESCC). In this study we aimed to study the role of HPV in ESCC.
Patients and Methods:In this study, 140 cases of ESCC were analyzed for the HPV DNA by polymerase chain reaction (PCR) using GP5+/GP6+ primers for L1 open reading frame (ORF) to amplify a 150-bp segment of HPV L1 ORF. This region was subsequently sequenced to identify the type of HPV.
Results:A total of 140 patients enrolled in our study. In this respect, 50.7% of them were females and 49.3% were males, aged between 20 and 81 years old. In addition, 33 tumor specimens (23.6%) and 12 (8.6%) non-involved tumor margins were HPV positive. In HPV positive tumor cases, 36% were also positive in tumor margins. The HPV positive cases were 21.7% males and 25.3% females. There was no correlation between the presence and types of HPV with patients’ sex and age. The frequency of HPV subtypes in tumoral regions were as follow: HPV-16: 60.6%, HPV-18: 30.3%, HPV-33: 6.1%, and HPV-31: 3 %. We found only HPV-16 in tumor margins.
Conclusion:Our results support a causal association between HPV infection and ESCC which is consistent with HPV studies conducted in other high-risk areas.
