Mohammad Reza Keramati; Mohammad Hadi Sadeghian; Hossein Ayatollahi; Houshang Rafatpanah; Mohammad Khajeh Daluei; Nafise Baesi
Volume 6, Issue 2 , April 2011, , Pages 56-62
Abstract
Background and Objectives: Complement proteins are some of the most important plasma proteins of the innate immune system. Impaired immune function is reported in subjects who are iron deficient, and there are documents that these patients are prone to infection. This study was conducted to show whether ...
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Background and Objectives: Complement proteins are some of the most important plasma proteins of the innate immune system. Impaired immune function is reported in subjects who are iron deficient, and there are documents that these patients are prone to infection. This study was conducted to show whether serum C3 and C4 complement change in adult nonpregnant female with iron deficient anemia or not. Methods: Forty five normal subjects and 45 iron deficient anemia (hypochrom microcytic) cases were entered in this case and control study by using patients’ clinical history and also results of CBC, Serum ferritin, iron and total iron binding capacity. Serum C3, and C4 were measured in case and control subjects with nephlometry method, finally comparison between result of patients group and control group was done with using suitable statistical test. Results: Mean serum C3 and C4 in patient group was 1.28 ± 0.81 and 0.28 ± 0.23 g/L respectively and for control group was 1.39 ± 0.87 and 0.35 ± 0.25 g/L respectively. Although serum complements were slightly lower in patient groups in compared to control group but this differences was not meaningful with t test. Conclusion: This study showed serum C3 and C4 complements levels were not changed in iron deficiency anemia.
Mehdi Seilanian Toosi; Jalil Tavakkol Afshar; Mohamad Reza Ghavam Nassiri; Mona Malekzadeh Moghani; Houshang Rafatpanah; Azam Brook
Volume 2, Issue 2 , April 2007, , Pages 59-66
Abstract
Background and Objective: Host genetic factors such as cytokine gene polymorphisms as well as Helicobacter pylori (H. pylori) infection have been found to be associated with gastric cancer risk . Interleukin 1 is a pro-inflammatory cytokine involved in H. pylori-induced gastric inflammation. Therefore, ...
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Background and Objective: Host genetic factors such as cytokine gene polymorphisms as well as Helicobacter pylori (H. pylori) infection have been found to be associated with gastric cancer risk . Interleukin 1 is a pro-inflammatory cytokine involved in H. pylori-induced gastric inflammation. Therefore, we analyzed the association between IL-1β and IL-1-RN polymorphisms and gastric cancer in Persian residents in north-eastern Iran. Methods: In a case-control study, the genotyping was carried out by PCR-RFLP in 109 gastric cancer patients and 101 randomly-selected healthy controls. The polymorphic sites include promoter region of IL-1β at 511 (C-T transition) position and IL-RN VNTR H. pylori infection was determined by ELISA assay in patients. Results: No significant differences were observed in the allele and genotype frequency of IL-1β-511 and IL-1RN VNTR between patients and control. Genotype frequencies in healthy controls were not significantly different from gastric cancer cases in separate histological types (intestinal or diffuse). IL- 1β-511 CT genotype frequency was significantly higher among healthy subjects than H. pylori positive gastric cancer patients (41.6% vs. 20%, p = 0.01, OR 0.30, 95% CI: 0.11-0.76). Meanwhile, relatively higher frequency of IL-1β-511 T genotype was observed among H. pylori positive cases as compared to healthy controls (42.9% vs. 26.7%, p = 0.06, OR 2.16, 95% CI: 0.96-4.8) Conclusion: Our results suggest the association between IL-1β-511 polymorphism and H. pylori infection and their contribution to the risk of gastric cancer. While IL-1β-511 CT genotype has a protective effect against H. pylori associated gastric carcinoma, IL-1β-511 TT may increase the risk.