Diagnostic Pathology
Hiva Saffar; Maryam Saatchi; Anahita Sadeghi; Fahimeh Asadi Amoli; Seyed Mohammad Tavangar; Farzaneh Shirani; Ali Aliasgari
Abstract
Background & Objective: Appropriate use of laboratory testing is essential for achieving safe and effective care to patient. Insufficient knowledge could lead to poor case management and increase the health care costs. It is believed that education on laboratory testing for undergraduate medical ...
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Background & Objective: Appropriate use of laboratory testing is essential for achieving safe and effective care to patient. Insufficient knowledge could lead to poor case management and increase the health care costs. It is believed that education on laboratory testing for undergraduate medical student is inadequate. This study was designed to evaluate the level of knowledge of 5 and 6 year undergraduate medical students in field of laboratory medicine. Methods: Totally, 59 questions including 8 basic questions concerning individual assessment of their knowledge and 51 objective questions focusing on various stages of test ordering and interpretation were asked. Result: Thirty seven undergraduate medical students at the level of internship participated. On average, 47.9% of students evaluated themselves as “weak" in 8 self-assessment questions. There was no significant difference between responders assessment on their own knowledge in various aspects of laboratory testing (P=0.184). In the objective questions regarding various stages of test ordering including pre-analytic, analytic and post analytic phases,45.6%,51.9% and 50% correct answers were reported ,respectively. Comparison of the level of the knowledge of the students regarding various stages of pathology testing did not show significant difference (P=0.638). Conclusion: Prioritizing an effective teaching method of laboratory medicine to medical students on appropriate time should be considered in medical school curriculum for better clinical decision making and optimal modern medical care.
Fahimeh Asadi Amoli; Masoumeh Mohebbi; Amir Hossein Sina
Volume 6, Issue 4 , September 2011, , Pages 224-228
Abstract
Chloroma or granulocytic sarcoma (GS) is a malignant neoplasm of myeloid lineage that occur in a variety of anatomic sites other than the bone marrow including soft tissue, bone, lymph node, nasal fossa, skin and sometime in the orbit. In the subconjunctiva it is rarely reported. A 4-year-old ...
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Chloroma or granulocytic sarcoma (GS) is a malignant neoplasm of myeloid lineage that occur in a variety of anatomic sites other than the bone marrow including soft tissue, bone, lymph node, nasal fossa, skin and sometime in the orbit. In the subconjunctiva it is rarely reported. A 4-year-old girl with a history of treated acute myelogenous leukemia was referred to Farabi Eye Hospital, Tehran, Iran for the evaluation of subconjunctival mass that was appeared since one month ago. Cell blood count (CBC) of the patient was normal except for Hb=7gm/dl. General physical examination was normal except for the signs of anemia. Histopathological evaluation of subconjunctival mass showed a malignant large round cell neoplasm. Immunohistochemical studies confirmed granulocytic sarcoma. When confronting a large cell or undifferentiated round cell neoplasm, the possibility of myeloid sarcoma should be investigated. Myeloperoxidase (MPO), cluster designation (CD) molecules such as CD34, CD45, CD15, CD68 and lysozyme positivity or Leder staining are useful in this regard.
Fahimeh Asadi Amoli; Ali Sadeghi Tarri; Khalil Hamzeh Doost; Naser Kamalian; Hedieh Moradi Tabriz
Volume 6, Issue 3 , June 2011, , Pages 124-132
Abstract
Background and Objectives: We aimed at evaluating the efficacy of fine needle aspiration biopsy (FNA) in comparison with histopathology and demonstrating whether cytological study could be a proper diagnostic tool in orbital mass lesions. Materials and Methods: In a cross sectional study during 36 months, ...
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Background and Objectives: We aimed at evaluating the efficacy of fine needle aspiration biopsy (FNA) in comparison with histopathology and demonstrating whether cytological study could be a proper diagnostic tool in orbital mass lesions. Materials and Methods: In a cross sectional study during 36 months, patients referred to our ophthalmologic center affiliated to Tehran University of Medical Sciences, for evaluation of orbital masses, were selected for FNA. After the surgery, the results of FNA were compared against histopathologic diagnoses as our gold standard method. Finally, the frequencies of specimen adequacy, the accuracy of FNA in distinguishing benign and malignant lesions and in the exact definitive diagnosis of the disorders were reported. Results: In 27.4% of the total 62 cases, the specimens were inadequate for cytologic evaluations. The rate of specimen adequacy in malignant and benign lesions was 82.6 % and 66.66%, respectively. From the morphologic point of view, the rate of the exact definitive diagnosis of malignant and benign disorders in the total 62 cases was 78.2% and 38.46% and; in the adequate specimens, it was 94.73% and 57.69%, respectively. There was no false positive FNA result for malignant cells and only in one malignant case, the FNA report was falsely negative. All data wee analyzed by SPSS software and p value Conclusion: FNA was considered more beneficial in the diagnosis of malignant lesions. FNA is a relatively noninvasive, rapid, specific, and accurate method for the preoperative primary diagnosis of orbital mass lesions and especially in malignant lesions and in some conditions, specific diagnoses can be achieved.
Fahimeh Asadi Amoli; Faezeh Foroughi; Hormoz Shams; Issa Jahanzad
Volume 5, Issue 2 , March 2010, , Pages 60-64
Abstract
Background and Objectives: Retinoblastoma is the most common intraocular pediatric malignancy. Angiogenic factor expression such as VEGF (vascular endothelial growth factor) in retinoblastoma can be confirmatory angiogenic potential of this tumor. This study was performed to determine the role ...
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Background and Objectives: Retinoblastoma is the most common intraocular pediatric malignancy. Angiogenic factor expression such as VEGF (vascular endothelial growth factor) in retinoblastoma can be confirmatory angiogenic potential of this tumor. This study was performed to determine the role of angiogenesis in local invasion of retinoblastoma and its correlation with patients’ survival. Materials and methods: This clinicopathological analysis was performed on 60 paraffin- embedded eyes with adequate tumoral tissue, which were stained using a CD34 antibody. Microvessel count was carried out in three tumor areas with the richest vascularity (hot spots) at a high magnification (× 400). The obtained data were correlated with histopathological characteristics and 5-years survival. Statistical analysis of the data was performed using student t-test and ANOVA test (P<0.05). Results: Tumor with local invasion to choroids, optic nerve, sclera and cilliary body showed statistically significant higher mean vessel density (P=0.00, P=0.041, P= 0.008 and P= 0.002, respectively). In addition, a statistically significant correlation was detected between mean vascular density and 5-years survival (P=0.031). Conclusion: The results suggest that in retinoblastoma, mean vessel density has a significant role in local invasion of tumor growth and a significant correlation with patient survival. Therefore, in these patients an anti-angiogenic therapy and minute diagnostic and follow up programs should be considered to identify metastasis.
Seyed Ali Ahmadi; Fahimeh Asadi Amoli; Katayoun Gohari Moghaddam
Volume 3, Issue 3 , June 2008, , Pages 151-156
Abstract
Background and Objective: The frequency and pattern of eye neoplasms differ in various geographic areas. Our aim was to determine the distribution of patient age, gender, ocular tumor origin, location and histological type in our region. Materials and Methods: In this cross-sectional study, ...
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Background and Objective: The frequency and pattern of eye neoplasms differ in various geographic areas. Our aim was to determine the distribution of patient age, gender, ocular tumor origin, location and histological type in our region. Materials and Methods: In this cross-sectional study, all of the 384 eye globe enucleation cases carried out during a 10-year period in Farabi Medical Center in Tehran were selected and reviewed. The inflammatory and non-tumoral conditions were excluded. Results: Out of 350 primary tumors, 344 (98.6%) were malignant including 248 cases of retinoblastomas (70.9%), 76 cases of (21.7%) melanomas and 14 cases of (4%) squamous cell carcinomas. Mean ages for these tumors were 3.2, 51 and 64.8 years respectively. Overall the tumors were 1.15 times more frequent in left eye and 1.53 times more common in males. Out of 248 cases of retinoblastomas, only 12 (4.8%) cases were bilateral (mean age 3.46 years; male to female ratio 1.4:1). Some rare cases in our series included a primary non-Hodgkin lymphoma of choroid, two choroidal leiomyomas and an adenocarcinoma of the retinal pigment epithelium. Conclusion: The overall frequency of the three most common primary malignancies in our series was similar to the reported Asian countries but slightly differed with western countries in terms of lower incidence of melanoma here. Also bilateral retinoblastoma was less frequent here and occurred in a slightly older age. The actual frequency of metastatic eye tumors could not be evaluated precisely due to lack of systematic autopsy data.
