Ghodsie Alavi; Nourieh Sharifi; Ali Sadeghian; Alireza Rezaei; Hossein Shidaee
Volume 7, Issue 3 , July 2012, , Pages 151-156
Abstract
Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, ...
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Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, some studies did not detect HPV DNA in ovarian carcinoma tissues. In this article, we investigated the potential role of high risk HPVs in the ovarian epithelial carcinoma.
Methods: Fifty archived epithelial ovarian cancer paraffin blocks were collected. Then, 30 non-malignant ovarian blocks used as control. These samples were histopathologically were confirmed by a pathologist and the proper blocks for DNA extraction and PCR were sorted. PCR was conducted deploying highly specific primers for high-risk types of HPV (18 and 16) according to the instructions of manufacturer company.
Results: High-risk oncogenic sequences were identified in 4 (5%) of the 80 studied samples. Of the 4 HPV positive cases, there was 1 case with normal tissue, 1 case of mucinous cyst adenocarcinoma, and 2 cases of serous cyst adenocarcinoma
Conclusion: Surprisingly, our findings could not support any association between high-risk oncogenic human papilloma virus (18 and 16) and malignant ovarian epithelial cancer. Therefore, that HPV is highly unlikely to play any causal role in the pathogenesis of epithelial ovarian neoplasia.
Zohreh Yousefi; Shohreh Saeed; Nourieh Sharifi
Volume 5, Issue 2 , March 2010, , Pages 105-108
Abstract
Placental site trophoblastic tumor (PSTT) is the rare variant of gestational trophoblastic diseases (GTD), which differs histologically and immunologically from GTD. Diagnosis of PSTT is often difficult and delayed. In most cases, diagnosis is not possible until the surgery is performed. The ...
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Placental site trophoblastic tumor (PSTT) is the rare variant of gestational trophoblastic diseases (GTD), which differs histologically and immunologically from GTD. Diagnosis of PSTT is often difficult and delayed. In most cases, diagnosis is not possible until the surgery is performed. The most therapeutic choice is hysterectomy and it is usually successful. Here we report two cases of PSTT.
N. Sharifi; M. Katebi
Volume 3, Issue 3 , June 2008, , Pages 146-150
Abstract
Background and Objective: Histological grading has prognostic and therapeutic implications in meningioma. However, histological criteria are sometimes not fulfilled to predict the biological behavior of meningiomas. P63 gene is a novel P53 family member, known as tumor suppressor factor, with ...
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Background and Objective: Histological grading has prognostic and therapeutic implications in meningioma. However, histological criteria are sometimes not fulfilled to predict the biological behavior of meningiomas. P63 gene is a novel P53 family member, known as tumor suppressor factor, with multiple isoforms and shows high expression in many normal and neoplastic human tissues. In this study, we investigated expression of P63 in different histological grades of meningiomas. Material and Methods: For this purpose, we studied and analyzed the immunohistochemical expression of P63 by a monoclonal antibody (clone 4A4) that recognizes all P63 isoforms in 52 cases of meningioma including WHO grade I (42 cases), grade II (7 cases) and grade III (3 cases). Correlation between histological grade and parameters like cell nuclear immunoreactivity to P63 antibody, age, gender and site of meningioma was analyzed by parametric and non-parametric statistical tests. Results: Among 52 patients analyzed, there were 35 females (67.3 %) and 17 males (32.7%) between 13 and 75 years old. Histological grading revealed 42 cases (80.8%) as WHO grade I, 7 (13.5%) as grade II and 3 (5.8%) cases were grade III. Expression of P63 protein was found in 83.2% of cases with grade I but in grade II and III, all of the cases were immunoreactive, so as in grade III, 100% of cases were with higher percent of nuclear reactivity in our study. Correlation between histological grade and nuclear immunoreactivity was highly significant (p=0.001). Conclusion: We conclude that the immunohistochemical staining of P63 will be valuable in discrimination of different grades of meningiomas.
Nourieh Sharifi; Mohammad Javad Yazdanpanah
Volume 2, Issue 3 , June 2007, , Pages 97-99
Abstract
Background and Objective: Cutaneous cytology is the study of scraping or smears obtained from skin lesions. Although not commonly employed, cutaneous cytology has been a useful tool for clinicians due to simplified procedures and high sensitivity and specificity of presurgical clinical diagnosis of common ...
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Background and Objective: Cutaneous cytology is the study of scraping or smears obtained from skin lesions. Although not commonly employed, cutaneous cytology has been a useful tool for clinicians due to simplified procedures and high sensitivity and specificity of presurgical clinical diagnosis of common types of skin tumors. This investigation was conducted to study usefulness of cutaneous cytology in diagnosis of basal and squamous cell carcinoma. Methods: We collected 42 skin tumors in total, clinically presumed to be either BCC (n = 31) or SCC (n = 10). Samples were taken by the scraping technique. After smearing the cells onto several glass slides, they were fixed with 96% alcohol. The specimens were stained with Papanicolaou stain. Punch biopsies were taken to confirm the clinical and cytologic impression. Results: Cytodiagnosis and histopathologic examination of 42 cases of BCC (n = 32) and SCC (n = 10) showed a significant concordance between histopathology of skin specimen and cytologic diagnosis with a sensitivity rate of 96.8% and specificity rate of 90% and with a high positive predictive value of 96.8%. Conclusion: Cytologic examination is easy to perform, saves time, and provides a rapid and reliable procedure in diagnosis and confirmation of non-melanoma malignant skin tumors (BCC and SCC).
