Mohammad Hossein Gheini; Noushin Jalayer Naderi
Abstract
Background & Objective: The role of synchronized expression pattern of cytokeratin (CK) 7 and CK20 in the prognosis of colon adenocarcinoma is unclear. The current study aimed at determining the relationship between the expression of cytokeratins 7 and 20 and prognostic factors in colon adenocarcinoma. ...
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Background & Objective: The role of synchronized expression pattern of cytokeratin (CK) 7 and CK20 in the prognosis of colon adenocarcinoma is unclear. The current study aimed at determining the relationship between the expression of cytokeratins 7 and 20 and prognostic factors in colon adenocarcinoma. Methods: In the current cross sectional Study, 52 archival samples of colon adenocarcinoma with different histopathologic differentiation were examined immunohistochemically to analyze the expression of Ck7 and Ck20. The relationship between cytokeratin expression and prognostic factors, such as histopathologic differentiation, lymph node involvement, and depth of invasion, were assessed. Results: CK7-/CK20+ was the most prevalent pattern in the current study. The difference among histopathologic grade, lymph node involvement, and depth of invasion in different CK7/CK20 expression patterns was insignificant (P=0.26, P=0.46, and P=0.22, respectively). Conclusion: No relationship was observed between CK7/CK20 expression and prognostic factors in colon adenocarcinoma, in the current study
Shamsa Shariatpanahi; Shahryar Pourfarzam; Mohammad hosein Gheini
Volume 11, Issue 3 , July 2016, , Pages 265-271
Abstract
Macrophage Activating Syndrome (MAS) is a life-threatening disease seen in autoimmune diseases including lupus erythematosus, rheumatoid arthritis, Still's disease, polyarteritis nodosa. It is characterized by fever, pancytopenia, liver failure, coagulopathy, and neurologic symptoms and high serum ferritin. ...
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Macrophage Activating Syndrome (MAS) is a life-threatening disease seen in autoimmune diseases including lupus erythematosus, rheumatoid arthritis, Still's disease, polyarteritis nodosa. It is characterized by fever, pancytopenia, liver failure, coagulopathy, and neurologic symptoms and high serum ferritin. A 27 yr. old female patient was admitted in shahid Mostafa Khomeini Hospital (Tehran-Iran) in May 2011 because of lower extremities edema and ascites and fever from 1.5 month ago. In physical examinations she had generalized lymphadenopathy, splenomegaly and pleural effusion. In laboratory tests she had pancytopenia, positive ANA and Anti DNA (ds), hypocomplementemia, hypertriglyceridemia and high ferritin level. Gradually she had signs of RPGN and ARDS. The patient had no skin and musculoskeletal signs of SLE and no liver failure nor coagulopathy of MAS. Her lymph node biopsy was reported as Castleman syndrome. Unlike other studies, the patient showed MAS before treatment with cytotoxic for lupus nephritis.
Mohammad Hossein Ghaini; Shaghayegh Sadat Esmailnejad; Ali Davati
Volume 7, Issue 3 , July 2012, , Pages 145-150
Abstract
Background and Aims: Immunohistochemical tests are one of the most important tests, which are under study to determine the prognosis of the cancers such as transitional cell carcinoma. By the time, flexible cystoscopy and urine cytology are the routine tests for following up the patients with transitional ...
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Background and Aims: Immunohistochemical tests are one of the most important tests, which are under study to determine the prognosis of the cancers such as transitional cell carcinoma. By the time, flexible cystoscopy and urine cytology are the routine tests for following up the patients with transitional cell carcinoma, which are both operator dependent. On the other hand, cystoscopy is an invasive method, and urine cytology is a method with low sensitivity. The aim of this study was to determine CK20 in patients with transitional cell carcinoma of bladder and its relation with prognostic factors, which are the stage and the grade of the tumor.
Materials and Methods: Our study was done in Mostafa Khomeini Hospital from 2007 to 2010 on the 2001 to 2009 stored information files, included 53 patients diagnosed as transitional cell carcinoma of bladder (TCC) of different stages and grades that were underwent total cystectomy. Immunohistochemical staining was performed on tissue sections with specific CK20 antibody. Then the samples were studied by light microscope so positive and negative cases were identified.
Results: According to statistical analysis there were significant reverse relationship between CK20 and stage, and significant reverse relationship between CK20 and grade (P= 0.000).
Conclusion: Immunohistochemical study of patients with transitional cell carcinoma of bladder in order to identify CK20 can be a useful method to determine the prognosis of these patients.
Alireza Azizzadeh Delshad; Marjan Heshmati; Mohammad Hossein Ghaini
Volume 5, Issue 3 , June 2010, , Pages 126-131
Abstract
Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material ...
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Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material and Methods: Human colon adenocarcinoma cells HT29 were treated with different doses (1, 5 and 10 mg/ml) of fresh garlic extract in cell culture at 24, 48 and 72 h. Cell death was assessed by viability test, and its apoptotic nature was determined by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay. All experiments were statistically analyzed using SPSS 11.5 software Results: Garlic extract induced a dose-dependent cell death with the highest values at 72 hours. The apoptosis analyses by TUNEL assay demonstrated that in each dose/time group the apoptotic values are much less prominent than the related cell death percentage found in viability test. In every dose, the sum of apoptotic cells in all three-time points was nearly equal to the percentage of cell death at 72 hours. Conclusion: Fresh garlic extract can induce a dose-dependent apoptotic cell death in cultured HT29 cell line.
Suzan Sanavi; Reza Afshar1; Mohammad Hossein Ghaini
Volume 3, Issue 3 , June 2008, , Pages 170-172
Abstract
A male patient with acute renal failure (ARF) due to large B-cell non-Hodgkin lymphoma infiltration of kidney is presented. The diagnosis was suspected because of coincidence of ARF and tumor lysis syndrome non-responsive to conservative renal therapies. A renal biopsy confirmed diagnosis and ...
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A male patient with acute renal failure (ARF) due to large B-cell non-Hodgkin lymphoma infiltration of kidney is presented. The diagnosis was suspected because of coincidence of ARF and tumor lysis syndrome non-responsive to conservative renal therapies. A renal biopsy confirmed diagnosis and appropriate chemotherapy led to complete improvement of renal function.
M.Hossein Ghaini; T Ghazanfari; N Fallah
Volume 1, Issue 1 , January 2006, , Pages 25-30
Abstract
Objective: Helicobacter pylori has a well-established role in the development of gastric cancer. In vitro studies reveal increased proliferation of the gastric mucosa in the presence of H. pylori infection. It has been also shown that production of some cytokines, such as interleukin-1 beta (IL-1b) is ...
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Objective: Helicobacter pylori has a well-established role in the development of gastric cancer. In vitro studies reveal increased proliferation of the gastric mucosa in the presence of H. pylori infection. It has been also shown that production of some cytokines, such as interleukin-1 beta (IL-1b) is increased in H. pylori infection. In addition, IL-1b increases proliferation of gastric epithelial cell in culture study. In this study, The effect of transforming growth factor beta (TGF-b) on gastric epithelial cell proliferation has been examined. Materials and methods: AGS cells were cultured with TGF-b. DNA synthesis was evaluated by bromo-deoxyuridine (BrdU) test and total viable cell numbers by MTT assay. Results: TGF-b decreased DNA synthesis and cell numbers. This effect was both timeand dose-dependent (p<0.05). Both tests, BrdU test & MTT assay revealed this suppressive effect, but it was more evident in BrdU test. Conclusion: TGF-b suppresses proliferation of malignant gastric epithelial cells. It appears that modulatin of tyrosine kinase activity is essential to anti-proliferative effect of TGF-b. Decreased gastric epithelial proliferation due to TGF-β may contribute to the decreased risk of gastric cancer and precancerous lesions in H. pylori-infected individuals or slowing the progression of disease in gastric cancer patients.
