Masood Saleem Mir; Mohammad Maqbool Darzi; Omer Khalil Baba; Hilal Musadiq Khan; Shayaib Ahmad Kamil; Asif Hassan Sofi; Sarfraz Ahmad Wani
Abstract
Background & Objectives: Streptozotocin (STZ) is used for induction of Type-1 diabetes mellitus in animal models. Its beta-cytotoxic action results in sudden release of insulin leading to severe hypoglycaemia and even mortality. However, its sensitivity varies with species. Present investigation ...
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Background & Objectives: Streptozotocin (STZ) is used for induction of Type-1 diabetes mellitus in animal models. Its beta-cytotoxic action results in sudden release of insulin leading to severe hypoglycaemia and even mortality. However, its sensitivity varies with species. Present investigation was aimed at studying STZ induced acute clinical effects in rabbits. Methods: Streptozotocin@ 65 mg/kg b.w.was administered to thirteenNew Zealand White rabbits, 1-1.5 kg body weight, as single intravenous dose in 1mL citrate buffer, pH 4.6. Blood glucose levels were recorded before drug administration and then at 20 min, 1h, and hourly up to 9 hours post-treatment followed by intravenous and oral glucose therapy. Clinical signs were noted. Results: STZ caused immediate hyperglycaemia up to 4 hours, and then progressively severe hypoglycaemia up to 9 hours. Hypoglycaemia caused characteristic behavioural alterations including lethargy, dullness, sitting quietly but appearing alert, followed by aesthesia and then muscular weakness with characteristic postural changes starting from drooping of head and torticollis, Rabbits recovered following glucose therapy. Marked individual variations in response vis-a-vis onset and severity of glycaemic changes were observed. Conclusion: STZ induced a characteristic multiphasic immediate response in rabbits similar to one reported in other rodents. Behavioural changes were characteristic of hypoglycaemia warranting early management in order to avoid fatalities. How to cite this article: Saleem Mir M, Maqbool Darzi M, Baba OK, Musadiq Khan H, Ahmad Kamil S, Sofi AH, et al. Streptozotocin Induced Acute Clinical Effectsin Rabbits (Oryctolagus cuniculus). Iran J Pathol. 2015;10(3):206-13.
Masood Saleem Mir; Mohammad Maqbool Darzi; Hilal Musadiq Khan; Shayaib Ahmad Kamil; Asif Hassan Sofi; Sarfaraz Ahmad Wani
Volume 9, Issue 2 , April 2014, , Pages 197-112
Abstract
Background & Objectives: Alloxan & streptozotocin are used for inducing diabetic models. Their combination has been used to reduce the individual chemical dosage and minimize the side effects. Present investigation was aimed at studying pre-diabetic clinical changes induced by low doses of Alloxan-STZ ...
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Background & Objectives: Alloxan & streptozotocin are used for inducing diabetic models. Their combination has been used to reduce the individual chemical dosage and minimize the side effects. Present investigation was aimed at studying pre-diabetic clinical changes induced by low doses of Alloxan-STZ cocktail in rabbits.
Methods: New Zealand White rabbits, 1-1.5 kg body weight, were administered alloxan (@50 mg/kg b.w.) and STZ (@ 35mg/kg b.w.) cocktail, as single intravenous dose. Blood glucose levels were monitored (0 h, 20 min, 1 h, and then hourly up to 9 h) and clinical signs noted. Rabbits surviving up to 9 hours were given glucose therapy.
Results: The cocktail caused immediate transient hypoglycaemia, followed by hyperglycaemia, and then progressively severe hypoglycaemia. Hypoglycaemia caused characteristic behavioural alterations from lethargy, through aesthesia, muscular weakness to recumbency. Severely affected rabbits revealed intermittent convulsions and died in coma.
Conclusion: Low dose Alloxan-STZ cocktail induced triphasic immediate response in rabbits. The behavioural changes reflected glycaemic status serving as a guide for institution of glucose therapy.