Breast Pathology
Armin Borhan; Zohreh Nozarian; Alireza Abdollahi; Reza Shahsiah; Hadiseh Mohammadpour; Arash Borhan
Abstract
Background & Objective: Nowadays, actin-binding proteins such as Villin and Gelsolin have been considered to be associated with aggressive tumors. This study mainly aims to determine the relationship between Gelsolin and Villin genes expression and metastasis of axillary lymph nodes in patients with ...
Read More
Background & Objective: Nowadays, actin-binding proteins such as Villin and Gelsolin have been considered to be associated with aggressive tumors. This study mainly aims to determine the relationship between Gelsolin and Villin genes expression and metastasis of axillary lymph nodes in patients with breast cancer.Methods: The included population consisted of 40 confirmed cases of female breast cancer (including 20 patients with breast cancer along with axillary lymph node metastasis and 20 patients without axillary lymph node metastasis). Expression of Villin and Gelsolin genes was evaluated using Real-time PCR and pre-designed primers.Results: The mean expression level of Villin in groups with and without axillary lymph node metastasis was 3.33±1.35 and 0.87±0.88, respectively (p <0.001). The mean Gelsolin expression levels in both groups (with and without axillary lymph node metastasis) were 4.13±2.40 and 1.00±0.35, respectively (p <0.001). The significant relationships were independent of individuals’ age.Conclusion: Patients with axillary lymph node metastasis may express significant higher level of Villin and Gelsolin gens.
Fatemeh Nili; Reza Shahsiah; Farid Azmoudeh Ardalan; Mohsen Nassiri Toosi; Alireza Abdollahi
Volume 9, Issue 1 , January 2014, , Pages 17-22
Abstract
Background and Objectives: HBV DNA monitoring is important in management of chronic viral hepatitis B infection. HBV DNA measurements are carried out over period of months to years. So the analytical system must be stable and reproducible. The aim of this study was to determine the performance ...
Read More
Background and Objectives: HBV DNA monitoring is important in management of chronic viral hepatitis B infection. HBV DNA measurements are carried out over period of months to years. So the analytical system must be stable and reproducible. The aim of this study was to determine the performance characteristics and to plan a statistical quality control system of a laboratory-developed real-time quantitative PCR assay for HBV DNA quantification. Methods: Values of systematic and random error at two clinical decision points;4.2 Log IU/mL (20000 IU/mL) and 3.2 Log IU/mL (2000 IU/mL) were determined. Candidate quality control procedures were selected and performance of the method by application of normalized operational process specification (OPSpecs) charts was determined. Results: The performance of the assay at level of 4.2 Log IU/mL and 3.2 Log IU/mL were excellent and good respectively. Moreover, a13.5S rule with two measurements offered 90% probability of error detection at level of 4.2 Log IU/mL, while no rule offered 90% probability of error detection at level of 3.2 Log IU/mL. Conclusion: Minimizing the formation of primer-dimer and nonspecific products and concentrating the target DNA during the purification process are proposed for accurate quantitative PCR particularly when CT values are high.
Reza Shahsiah; Maryam Monajemzadeh; Hasan Hoseinzadeh; Mohammad Taghi Haghi ashtiani; Sedigheh Hantooshzadeh; Alireza Alaei Alamooti; Fatemeh Mahjoub
Volume 6, Issue 4 , September 2011, , Pages 202-207
Abstract
Background and Objectives: Infection with parvovirus may induce spontaneous abortion, non-immune hydrops fetalis, and intrauterine death in full term infants. The aim of this study was to determine the frequency of parvovirus B19 in paraffin-embedded formalin fixed placental tissues in lost fetuses ...
Read More
Background and Objectives: Infection with parvovirus may induce spontaneous abortion, non-immune hydrops fetalis, and intrauterine death in full term infants. The aim of this study was to determine the frequency of parvovirus B19 in paraffin-embedded formalin fixed placental tissues in lost fetuses by PCR method and comparison with its frequency in healthy full term neonates as controls.
Materials and Methods: In a case control study, thirty-one formalin fixed paraffin embedded placental tissue from autopsies related to fetal losses and also the same number of control cases were collected and The PCR for internal control and target sequence were performed.
Results: Thirty-one subjects and 31 controls were enrolled and three out of 31 cases were positive for parvovirus (9.7%) using PCR. Hydropic changes were noted in two of positive cases for parvovirus.
Conclusion: Parvovirus B19 infection during pregnancy may cause red precursor cells damage resulting in anemia and congestive heart failure. We suggest that studies with greater sample sizes are carried out to determine the frequency and management of parvovirus B19 infection because of timely diagnosis and transfusion of severe hydropic fetuses can reduce the risk of fetal death
Alireza Abdollahi; Reza Shahsiah; Mohssen Nassiri Toosi; Manije Lak; Katayoun Karimi; Mohammadreza Managhchi; Katayoun Samimi-rad
Volume 3, Issue 3 , June 2008, , Pages 119-124
Abstract
Background and Objective: Although transfusion therapy has lead to great improvement in longevity for hemophiliacs, but there have been tragic setbacks especially from transmission of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) .HIV was reported to increase the rate of HCV-related ...
Read More
Background and Objective: Although transfusion therapy has lead to great improvement in longevity for hemophiliacs, but there have been tragic setbacks especially from transmission of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) .HIV was reported to increase the rate of HCV-related liver failure by 4.2 times. In this study, we aimed to determine the seroprevalence of HIV and HCV, the association of HCV with abnormal liver tests, impact of HIV on HCV-related abnormalities and the distribution of HCV genotypes in Iranian hemophiliacs. Patients and Methods: In a cross-sectional study, we determined virological, clinical and epidemiological characteristics for HIV and HCV infection of 236 hemophiliacs attending our center. Data were analyzed using Chi-square test. Results: Ten (4.7%) out of 211 patients tested were HIV seropositive and 145 (83.3%) were HCV seropositive. All tested positive HIV patients also had HCV. HCV seroprevalence was significantly higher in patients with hemophilia A and B as compared to other congenital coagulopathies and it was directly related to coagulation severity. HCV seroprevalence was lower in hemophiliacs with positive HBsAg (p = 0.03) but it did not differ by HBcAb or HBsAb results. HCV genotype 1a (48.5%) was predominant type and genotype 3a (33.3%) was also common. Frequency of abnormal aspartate aminotransferase and alanine aminotransferase liver enzymes was significantly higher in the HCV positive group (p = 0.006). Conclusion: This study provides evidence that hepatitis c virus infection is a major problem for Iranian hemophiliacs and it has higher prevalence in hemophiliacs with higher age, more severe coagulopathies, abnormal alanine aminotransferase level, and human immunodeficiency virus co-infection.
