Microbiology
Kosar Jalalvand; Nasrin Shayanfar; Freshteh Shahcheraghi; Elahe Amini; Masha Mohammadpour; Pegah Babaheidarian
Abstract
Background & Objective: Carbapenem-resistant Enterobacteriaceae is a growing concern worldwide including Iran. The emergence of this pathogen is worrying as carbapenem is one of the 'last-line' antibiotics for treatment of infections caused by multi drug resistant gram- negative bacteria. The main ...
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Background & Objective: Carbapenem-resistant Enterobacteriaceae is a growing concern worldwide including Iran. The emergence of this pathogen is worrying as carbapenem is one of the 'last-line' antibiotics for treatment of infections caused by multi drug resistant gram- negative bacteria. The main objective of this study was to determine the prevalence of carbapenem-resistant Enterobacteriaceae in a referral hospital in Tehran, Iran. Methods: In this study, all positive isolates of Enterobacteriaceae recorded in blood, urine, and other body fluids were studied during April 2017 to April 2018 in a referral hospital in Tehran. All cases of resistance to carbapenems were first tested by modified Hodge test. All cases with positive or negative test, after gene extraction, were examined genotypically based on the primers designed for the three Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-β-lactamase (NDM), and OXA-48 genes by conventional PCR method. Result: 108 isolates (13.6%) were resistant to all cephalosporins as well as to imipenem and meropenem. In a genotypic study, including 45 isolates, 13 isolates were positive for OXA-48 gene, 11 isolates for OXA-48 and NDM genes, 11 isolates for OXA-48, NDM and KPC genes, 4 isolates for OXA-48 genes and KPC, 3 isolates for NDM, one isolate for KPC. On the other hand, two isolates were negative for all three genes examined. Conclusion: OXA-48 gene was one of the most common genes resistant to carbapenems in Iran. According to studies, the prevalence of antibiotic resistance in Iran is rising dramatically, which reduces the choice of antibiotics to treat severe infections in the future.
Alireza Sadeghipour; Navid Abdi; Pegah Babaheidarian
Abstract
Background: Tuberous sclerosis (TSC) is inherited as an autosomal dominant disease, characterized by skin lesion and tubers in vital organs, especially brain in three categories including subependymal nodules, cortical tubers and subependymal giant cell astrocytoma. Subependymal giant cell astrocytoma ...
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Background: Tuberous sclerosis (TSC) is inherited as an autosomal dominant disease, characterized by skin lesion and tubers in vital organs, especially brain in three categories including subependymal nodules, cortical tubers and subependymal giant cell astrocytoma. Subependymal giant cell astrocytoma (SEGA) is an indolent neoplasm which usually arises at the cauda thalamic groove near foramen monro, although it occurs usually in the clinical settings of TSC, a few number of SEGA has been reported without such history. Its morphology with special cytoarchitecture could be mistaken with other glial brain tumors with similar morphology. Therefore, investigating new markers for differentiating SEGA from other mimickers seems logical rather than other glioneural immunohistochemical markers introduced before. Case: We investigated CD99 expression in SEGA as an adjunctive marker for diagnostic purposes. Five reported cases of SEGA were studied and all of them showed CD99 expression besides usual glioneural markers. Conclusion: CD99 may be a useful adjunctive marker in differentiating SEGA from other mimickers
Forough Hashemi; Pegah Babaheidarian; Soheila Dabiran
Abstract
Background: Meningioma is among the most common slow growing central nervous neoplasms, which recurs locally despite the benign histologic features. The aim of this study was to evaluate osteopontin and ki67 expressions in different histologic grades of meningioma in a group of Iranian people. Methods: ...
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Background: Meningioma is among the most common slow growing central nervous neoplasms, which recurs locally despite the benign histologic features. The aim of this study was to evaluate osteopontin and ki67 expressions in different histologic grades of meningioma in a group of Iranian people. Methods: In this cross-sectional study, first, the paraffin blocks with the definite pathological diagnosis of meningioma in 70 patients were cut by microtome, in 4-5 micron sizes and stained for immunohistochemical markers of osteopontin and ki67. Then, all samples were evaluated for positive immunoreactivity and contributing factors. Results: Among 70 pateints studied, the mean for the Ki67 level was 8.6±12.3 and the mean for the osteopontinm IHC score was 45.2±77.9. There was a high correlation between markers, tumor recurrence and grade (P<0.001). In addition, there was a significant direct correlation between ki67 and osteopontin levels (P<0.001, r=0.760). In other words, as OPN and ki67 expressions increased, the chance of tumor recurrence increased. Conclusion: ki67 and osteopontin expressions in patients with meningioma can be used as good prognostic markers for tumor recurrence and for distinguishing the grade of meningiomas.
How to cite this article: Hashemi F, Babaheidarian P, Dabiran S. Are Osteopontin and Ki67 Expressions Different in Various Histologic Grades of Meningioma? An Iranian Experience. Iran J Pathol. 2015; 10(4): 253 - 257.
Maryam Kadivar; Pegah Babaheidarian; Ali Zare Mirzae; Mohammadreza Fakhraie
Volume 8, Issue 2 , April 2013, , Pages 115-118
Abstract
There are few reported cases of endobronchial metastasis of pheochromocytoma literature. We present here a 56-year old woman who underwent left lower lobectomy of lung, following pneumonia with unresolved chest radiographs. Computed tomography showed a lobulated soft tissue mass, measuring, 38×27 ...
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There are few reported cases of endobronchial metastasis of pheochromocytoma literature. We present here a 56-year old woman who underwent left lower lobectomy of lung, following pneumonia with unresolved chest radiographs. Computed tomography showed a lobulated soft tissue mass, measuring, 38×27 mm, at the perivascular space of anterior mediastinum. The resected specimen, showed lobulated tumor arranged in nesting pattern within arcuate vascular network. Immunohistochemistry showed intense positive staining of epitheloid cell (chief cells) for chromogranin and synaptophysin which were negative for cytokeratin. Sustentacular cells were strongly positive for S-100. Although very rare, physicians should keep in mind the possibility of endobronchial metastasis in patients with a history of pheochromocytoma.
Ali Zare Mirzaie; Maryam Abolhasani; Roozbeh Mobasher Moghaddam; Maryam Kadivar
Volume 7, Issue 3 , July 2012, , Pages 183-189
Abstract
Background and Objectives: Gastrointestinal polyps are proliferative or neoplastic mucosal lesions. The most important point about these polyps is risk of malignancy of them. This study was performed to determine type and frequency of polyps of gastrointestinal tract in Iranian population according to ...
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Background and Objectives: Gastrointestinal polyps are proliferative or neoplastic mucosal lesions. The most important point about these polyps is risk of malignancy of them. This study was performed to determine type and frequency of polyps of gastrointestinal tract in Iranian population according to their locations.
Materials and Methods: Totally, 210 patients referred to Rasul-e-Akram Hospital in years 2006-2010 and had pathology report of gastrointestinal polyps were included in the study. Frequency of gastrointestinal polyps was determined according to type, histological subtype, location, age and sex. The data was analyzed by software SPSS 16.
Results: Of participants, 129 patients were male (61.4%) and 81 (38.6%) were female. The mean age of patients was 58.4±32 yr. The mode of age interval was 70-80 yr (25.2%). The most frequent presenting symptom was lower gastrointestinal bleeding as melena or hematochesia (31%). Colon and sigmoid were site of most of gastrointestinal polyps (74.2%). The most prevalent type of gastrointestinal polyps was adenomatous polyp which was reported in 175 patients (84.3%). The most common types of colonic and gastric polyps were adenomatous and hyperplastic types respectively.
Conclusion: Our data is highly confirmatory to previous studies regarding association of polyp with advanced age and male sex, the most prevalent symptom and site of gastrointestinal polyps, and the most common types of colonic polyps. The frequency of gastric polyps in our population differs with some studies.