Biology & Genetic
Payam Behzadi
Abstract
Dear EditorOn July 21 (Sunday) and 23 (Tuesday) 2019, I had an opportunity to hold another workshop titled “How to write a scientific paper?” for Ph.D. students in Microbiology Department, Basic Sciences Faculty, the Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran. I received brilliant ...
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Dear EditorOn July 21 (Sunday) and 23 (Tuesday) 2019, I had an opportunity to hold another workshop titled “How to write a scientific paper?” for Ph.D. students in Microbiology Department, Basic Sciences Faculty, the Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran. I received brilliant feedback from the participants encouraging me to publish this letter to the editor.The difficult work of writing is an art like rasping and grinding a rough diamond to have a polished and smooth diamond (1) or like making a film (2). Publishing peer-reviewed papers is a criterion for evaluating academic professionals and is an effective means of forming an academic career and resume (3).As we know since the ancient era, the published texts are used as public official means to communicate with diverse people and populations. According to the latest archeological studies, the first handwriting belongs to 10400 years ago discovered in Baluchestan Province, the southeast of Iran (4).The primitive means of scripts have evolved and now we have high-tech tools to publish different types of scripts as softcopies and hardcopies, as well as traditional and online publications (4, 5). English as an international language may connect all people around the world. Therefore, publishing scholarly papers in English language journals promotes the visibility and citation of the papers (5, 6).A strong scientific paper needs a clear roadmap, a well-designed study, and an up-to-date quality proposal. Blurry hypothesis, poor-designed study, biases, inappropriate sample size (e.g., very small and limited populations or samples), and wrong or insufficient statistical analyses are the most common reasons for the rejection of papers (6-8).I as a non-native English-speaking author and reviewer believe that the non-native English-speaking authors should think, imagine, and write their manuscripts in English. Many young non-native English-speaking authors write their manuscripts in their native language and then translate into English. This is very harmful because it makes your manuscript of poor quality in academic English language, results in more probability of rejection (7). Consequently, in this challenge, the authors should prepare a well-planned manuscript with a strong and clear hypothesis (it goes back to the proposal) and relevant aims. Moreover, proper statistical analyses and calculations, correspondent methodology, appropriate and precise conclusion, sharp and clear figures, and well-designed and self-explanatory tables augment the opportunity of a manuscript for acceptance (Figure 1) (4, 6, 7, 9, 10). AcknowledgementsI have special thanks to Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran for approving the perfomance of the workshop on “How to write a scientific paper?”. Conflict of InterestThe authors confirm that there are no known conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influenced its outcome.
Microbiology
Reza Ranjbar; Afsar Tabatabaee; Payam Behzadi; Rohollah Kheiri
Abstract
Background: Escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. Despite several advanced molecular-genomic technologies for detecting and identifying different strains of E. coli, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction ...
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Background: Escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. Despite several advanced molecular-genomic technologies for detecting and identifying different strains of E. coli, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) technique is a quick, sharp and cost effective fingerprint method. The major purpose of the present study was to determine the distribution of ERICs within E. coli strains isolated from different healthy animal stool specimens including hens, sheep, and cows, as an appropriate and quick molecular-genomic tool. Methods: The animal stool samples were obtained during 1 year (October 2012 to October 2013), from animal husbandries around Tehran and Alborz provinces, Iran. After screening processes, the E. coli bacteria were isolated and cultured via standard microbiological methods. The DNA molecules of E. coli bacteria were harvested and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was applied for bacterial molecular genotyping. The ERIC-PCR products were run on 1% gel electrophoresis. The final images regarding gel electrophoresis banding patterns were used for dendrogram generation via the GelClust software. Results: Of 120 isolated samples, 115 different strains were recognized as E. coli. The fingerprint patterns involved 380 to 3280 bp bands. The predominant bands included 2900 bp, 1200 bp, and 1200 bp in stool samples of hens, sheep, and cows, respectively. The highest frequencies and diversities were seen among E. coli strains isolated from hens and sheep stool samples. Conclusion: The DNA profiles were clearly detectable via specific fingerprint patterns. The ERIC-PCR seemed to be a good approach for molecular typing of E. coli strains isolated from different animal sources.
